中国农业科学 ›› 2009, Vol. 42 ›› Issue (8): 2862-2874 .doi: 10.3864/j.issn.0578-1752.2009.08.027

• 园艺 • 上一篇    下一篇

生物技术与葡萄遗传育种

王军   

  1. (东北林业大学林学院/林木遗传育种与生物技术教育部重点实验室)
  • 收稿日期:2008-11-14 修回日期:2008-12-18 出版日期:2009-08-10 发布日期:2009-08-10

Biotechnology and Genetic Breeding of Grapevine

WANG Jun   

  1. (东北林业大学林学院/林木遗传育种与生物技术教育部重点实验室)
  • Received:2008-11-14 Revised:2008-12-18 Online:2009-08-10 Published:2009-08-10

摘要:

生物技术的发展和应用已渗透到葡萄遗传育种的各个领域。DNA分子标记为研究葡萄品种起源和育成新品种提供了强有力的工具,特别是SSR标记在葡萄品种鉴别、系谱分析和遗传图谱构建方面已得到广泛应用。基于DNA分子标记遗传图的构建加速了杂种的早期选择,遗传图和物理图的结合可以克隆控制重要性状的功能基因,遗传转化技术的应用可以改良现有的葡萄品种。因此,生物技术正深刻改变着传统的葡萄育种方法。

关键词: 葡萄, 育种, DNA标记, 遗传图, 遗传转化

Abstract:

The development and application of biotechnology are permeating genetic breeding of grapevine as a whole. DNA markers facilitate investigations into the origins of existing cultivars and provide powerful tools for the creation of new cultivars. In particular SSR marker, is widely used in identifying grape cultivars, pedigree analysis, and constructing genetic map. DNA molecular marker-based genetic maps are accelerating grape breeding programs by permitting early selection for promising seedlings. Genetic mapping in combination with physical mapping can lead to the isolation of grape genes that control important traits. The application of technology in genetic transformation permits the targeted modification of existing grape cultivars. Therefore, biotechnology is dramatically changing the traditional methods of grapevine breeding today.

Key words: grapevine cultivars, breeding, DNA markers, genetic map, genetic transformation