中国农业科学 ›› 2008, Vol. 41 ›› Issue (4): 1165-1171 .doi: 10.3864/j.issn.0578-1752.2008.04.028

• 贮藏·保鲜·加工 • 上一篇    下一篇

茉莉酸甲酯诱导的采后香蕉果实耐冷性与活性氧信号的关系

王海波,黄椿颖,庞学群,徐兰英,张昭其   

  1. 华南农业大学园艺学院
  • 收稿日期:2007-07-16 修回日期:2007-10-12 出版日期:2008-04-10 发布日期:2008-04-10
  • 通讯作者: 张昭其

Methyl Jasmonate Induced Cold Resistance and Reactive Oxygen Species Signaling in Postharvest Banana

  1. 华南农业大学园艺学院
  • Received:2007-07-16 Revised:2007-10-12 Online:2008-04-10 Published:2008-04-10

摘要: 【目的】探讨茉莉酸甲酯(MeJA)对采后香蕉果实耐冷诱导的效果及其对活性氧代谢、Ca2+-ATPase活性的影响。【方法】香蕉果皮用10 µmol•L-1 MeJA处理1 min,在20℃恒温培养1 d(取样作第0天),然后置于7℃下离体培养10 d,测定果皮冷害指数、细胞膜透性、过氧化氢和超氧阴离子含量、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、NADPH氧化酶以及Ca2+-ATPase活性的变化。【结果】与对照香蕉果皮相比,10 µmol•L-1 MeJA处理的冷害指数较低、细胞膜透性增加缓慢;MeJA处理诱导了果皮H2O2含量和 产生速率的提高,并维持了前4 d的高水平,而MeJA处理降低了CAT和APX的活性,并在前4 d维持了较低的水平;研究还发现,MeJA处理还诱导了NADPH氧化酶和Ca2+-ATPase活性的升高。【结论】MeJA诱导的活性氧猝发可能作为信号分子参与诱导了香蕉果皮冷害和耐冷诱导,并可能与钙信号相关。

关键词: 茉莉酸甲酯, 冷害, 活性氧, 香蕉果皮

Abstract: Abstract: 【Objective】 The effects of methyl jasmonate (MJ) on cold resistance, reactive oxygen species (ROS) metabolism and Ca2+-ATPase activity of postharvest banana fruit were investigated in this paper. 【Method】 Banana peels were treated with 10 µM MJ and incubated in vitro at 7 ℃ for 10 days. The chilling injury index, cell membrane permeability, content of H2O2 and O2-, activity changes of CAT, APX, NADPH oxidase and Ca2+-ATPase were determined.【Result】Compared to control peels, the chilling injury index of MJ treated peels was less and the increase of cell membrane permeability was slower. Moreover, MJ treatment induced the increase of H2O2 content and O2- production and maintained their high levels before 4 days. However, MJ treatment reduced the activities of CAT and APX and maintained low activity before 4 days. MJ treatment was also found to induce the activities of NADPH oxidase and Ca2+-ATPase of banana peels.【Conclusion】The reactive oxygen species induced by MJ might serve as signal moleculars and be involved in chilling injury and chilling resistance of banana peels, and they might also induce calcium signaling. Key words: Methyl jasmonate ; Chilling injury; Reactive oxygen species; Banana peel