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| Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.) |
| ZHAO Zheng-wu, LUO An-cai, LE Tao , LI Shi-gui |
1.School of Life Sciences, Chongqing Normal University, Chongqing 400047, P.R.China
2.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China |
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摘要 312 recombinant inbred lines (RILs) in F9 from a cross between a overwintering cold-tolerant germplasm resource Glutinous rice 89-1(Gr 89-1) and a cold-sensitive variety Shuhui 527 was used for quantitative trait locus (QTL) analysis. The scores of percent ratooning germinability (PRG) and overwintering germinability (POG) were evaluated. The overwintering germination rate of axillary buds was scored to represent the overwintering germinability. Two significant QTLs (qPRG-4 and qPRG-7) on chromosomes 4 and 7 were detected and explained 8.3 and 7.2% of the total phenotypic variation, respectively. Three significant QTLs (qPOG-2, qPOG-3 and qPOG-7) were identified and mapped on chromosomes 2, 3, and 7, respectively. These QTLs contributed 9.6, 6.7, and 17.8% of phenotypic variations, respectively. A comparative analysis using SSR markers closely linked to the three QTLs for the overwintering revealed cold-tolerant individuals, which harbour the Glutinous rice 89-1 alleles at RM7110, RM250, RM418, and RM232, had a high percent overwintering germinability, while cold-sensitive individuals, which carry Shuhui 527 alleles at these loci, had a low percent overwintering germinability in the F2 population of Shuhui 527/Glutinous rice 89-1. This study demonstrated the utility of these SSR markers for selection of overwintering germinability genotypes.
Abstract 312 recombinant inbred lines (RILs) in F9 from a cross between a overwintering cold-tolerant germplasm resource Glutinous rice 89-1(Gr 89-1) and a cold-sensitive variety Shuhui 527 was used for quantitative trait locus (QTL) analysis. The scores of percent ratooning germinability (PRG) and overwintering germinability (POG) were evaluated. The overwintering germination rate of axillary buds was scored to represent the overwintering germinability. Two significant QTLs (qPRG-4 and qPRG-7) on chromosomes 4 and 7 were detected and explained 8.3 and 7.2% of the total phenotypic variation, respectively. Three significant QTLs (qPOG-2, qPOG-3 and qPOG-7) were identified and mapped on chromosomes 2, 3, and 7, respectively. These QTLs contributed 9.6, 6.7, and 17.8% of phenotypic variations, respectively. A comparative analysis using SSR markers closely linked to the three QTLs for the overwintering revealed cold-tolerant individuals, which harbour the Glutinous rice 89-1 alleles at RM7110, RM250, RM418, and RM232, had a high percent overwintering germinability, while cold-sensitive individuals, which carry Shuhui 527 alleles at these loci, had a low percent overwintering germinability in the F2 population of Shuhui 527/Glutinous rice 89-1. This study demonstrated the utility of these SSR markers for selection of overwintering germinability genotypes.
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Received: 19 July 2011
Accepted:
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| Fund: This research was supported by the Rice Key Project of Chongqing, China (CSTC2011AB1076), the Foundation Project of Chongqing Education Commission (KJ110623), the Doctor Foundation of Chongqing Normal University, China (10LR028) and the Plants Breeding Project of Chongqing, China (CSTC2010AA1033). |
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Corresponding Authors:
Correspondence LI Shi-gui, Tel: +86-28-82722661, E-mail: lishigui@263.com
E-mail: lishigui@263.com
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| About author: ZHAO Zhen-wu, Tel: +86-23-65910315, E-mail: zhaozhengwu513@sina.com |
Cite this article:
ZHAO Zheng-wu, LUOAn-cai , LE Tao , LI Shi-gui.
2012.
Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.). Journal of Integrative Agriculture, 12(11): 1767-1774.
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