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| Allelic Variation at the TaZds-A1 Locus on Wheat Chromosome 2A and Development of a Functional Marker in Common Wheat |
| DONG Chang-hai, MA Zhi-ying, ZHANG Li-ping |
1.College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China
2.Institute of Crop Sciences, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, P.R.China
3.CIMMYT China Office/CAAS, Beijing 100081, P.R.China
4.Beijing Engineering and Technique Research Center of Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, P.R.China |
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摘要 (zeta)-carotene desaturase (ZDS) is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment (YP) content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame (ORF) of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-A1a and TaZds-A1b, yielding 183- and 179-bp fragments in TaZds-A1a and TaZds-A1b genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid (DH) population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-A1b allele was 7.8% higher than that of 91 cultivars with TaZds- A1a allele.
Abstract (zeta)-carotene desaturase (ZDS) is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment (YP) content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame (ORF) of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-A1a and TaZds-A1b, yielding 183- and 179-bp fragments in TaZds-A1a and TaZds-A1b genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid (DH) population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-A1b allele was 7.8% higher than that of 91 cultivars with TaZds- A1a allele.
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Received: 25 February 2011
Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (30871522 and 30830072), the National Basic Research Program of China (2009CB118300), and an Earmarked Fund for the Modern Agro-Industry Technology Research System, China (CARS-3-1-3). |
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Corresponding Authors:
MA Zhi-ying, Tel: +86-312-7521590, E-mail: mzhy@hebau.edu.cn; HE Zhong-hu, Tel: +86-10-82108547, Fax: +86-10-82108547, E-mail:zhhecaas@163.com
E-mail: mzhy@hebau.edu.cn
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Cite this article:
DONG Chang-hai, MA Zhi-ying, ZHANG Li-ping.
2012.
Allelic Variation at the TaZds-A1 Locus on Wheat Chromosome 2A and Development of a Functional Marker in Common Wheat. Journal of Integrative Agriculture, 12(7): 1067-1074.
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