|
|
|
|
|
| Isolation and Expression Patterns of Rice WRKY82 Transcription Factor Gene Responsive to Both Biotic and Abiotic Stresses |
| PENG Xi-xu, TANG Xin-ke, ZHOU Ping-lan, HU Yao-jun, DENG Xiao-bo, HE Yan and WANG Hai-hua |
| School of Life Sciences, Hunan University of Science and Technology, Xiangtan 411201, P.R.China |
|
|
|
摘要 WRKY transcription factors are involved in the regulation of response to biotic and abiotic stresses in plants. A fulllength cDNA clone of rice WRKY82 gene (OsWRKY82) was isolated from a cDNA library generated from leaves infected by Magnaporthe grisea. OsWRKY82 contained an entire open reading frame in length of 1 701 bp, and was predicted to encode a polypeptide of 566 amino acid residues consisting of two WRKY domains, each with a zinc finger motif of C2H2,belonging to the WRKY subgroup I. OsWRKY82 shared high identity at the amino acid level with those from Sorghum bicolor, Hordeum vulgare, and Zea mays. The transcript level of OsWRKY82 was relatively higher in stems, leaves, and flowers, and less abundant in grains. It was induced by inoculation with M. grisea and Rhizoctonia solani. However, the inducible expression in incompatible rice-M. grisea interactions was earlier and greater than that in compatible interactions.The expression of OsWRKY82 was up-regulated by methyl jasmonate and ethephon, whereas salicylic acid exerted no effects on its expression. Moreover, OsWRKY82 exhibited transcriptional activation ability in yeast. Additionally,OsWRKY82 transcripts could be induced by wounding and heat shocking, but not by abscisic acid, cold, high salinity and dehydration. By contrast, gibberellin suppressed the expression of OsWRKY82. These indicate that OsWRKY82 is a multiply stress-inducible gene responding to both biotic and abiotic stresses, and may be involved in the regulation of defense response to pathogens and tolerance against abiotic stresses by jasmonic acid/ethylene-dependent signaling pathway.
Abstract WRKY transcription factors are involved in the regulation of response to biotic and abiotic stresses in plants. A fulllength cDNA clone of rice WRKY82 gene (OsWRKY82) was isolated from a cDNA library generated from leaves infected by Magnaporthe grisea. OsWRKY82 contained an entire open reading frame in length of 1 701 bp, and was predicted to encode a polypeptide of 566 amino acid residues consisting of two WRKY domains, each with a zinc finger motif of C2H2,belonging to the WRKY subgroup I. OsWRKY82 shared high identity at the amino acid level with those from Sorghum bicolor, Hordeum vulgare, and Zea mays. The transcript level of OsWRKY82 was relatively higher in stems, leaves, and flowers, and less abundant in grains. It was induced by inoculation with M. grisea and Rhizoctonia solani. However, the inducible expression in incompatible rice-M. grisea interactions was earlier and greater than that in compatible interactions.The expression of OsWRKY82 was up-regulated by methyl jasmonate and ethephon, whereas salicylic acid exerted no effects on its expression. Moreover, OsWRKY82 exhibited transcriptional activation ability in yeast. Additionally,OsWRKY82 transcripts could be induced by wounding and heat shocking, but not by abscisic acid, cold, high salinity and dehydration. By contrast, gibberellin suppressed the expression of OsWRKY82. These indicate that OsWRKY82 is a multiply stress-inducible gene responding to both biotic and abiotic stresses, and may be involved in the regulation of defense response to pathogens and tolerance against abiotic stresses by jasmonic acid/ethylene-dependent signaling pathway.
|
|
Received: 10 June 2011
Online: 10 June 2011
Accepted:
|
|
Corresponding Authors:
WAHNG Hai-hua
E-mail: pengxix@163.com;haihuawxt@163.com
|
| About author: PENG Xi-xu, MSc, Tel: +86-731-58291416, E-mail: pengxix@163.com; Correspondence WAHNG Hai-hua, Professor, Ph D, Tel: +86-731-58290476, Fax: +86-731-
58290478, E-mail: haihuawxt@163.com |
Cite this article:
PENG Xi-xu, TANG Xin-ke, ZHOU Ping-lan, HU Yao-jun, DENG Xiao-bo, HE Yan and WANG Hai-hua.
2011.
Isolation and Expression Patterns of Rice WRKY82 Transcription Factor Gene Responsive to Both Biotic and Abiotic Stresses. Journal of Integrative Agriculture, 10(6): 893-901.
|
| Bari R, Jones J D. 2009. Role of plant hormones in plant defence responses. Plant Molecular Biology, 69, 473-488. Chen C, Chen Z. 2000. Isolation and characterization of two pathogen- and salicylic acid-induced genes encoding WRKYDNA binding proteins from tobacco. Plant Molecular Biology, 42, 387-396. Chen W, Provart N J, Glazebrook J, Katagiri F, Chang H S, Eulgem T, Mauch F, Luan S, Zou G, Whitham S A, et al. 2002. Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses. The Plant Cell, 14, 559-574. Dong J, Chen C, Chen Z. 2003. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Molecular Biology, 51, 21-37. Eulgem T, Rushton P J, Robatzek S, Somssich I E. 2000. The WRKY superfamily of plant transcription factors. Trends in Plant Science, 5, 199-206. Eulgem T, Somssich I E. 2007. Networks of WRKY transcription factors in defense signaling. Current Opinion in Plant Biology, 10, 366-371. Hammond-Kosack K E, Parker J E. 2003. Deciphering plant pathogen communication: fresh perspectives for molecular resistance breeding. Current Opinion in Biotechnology, 14, 177-193. Jiang Y, Deyholos M K. 2009. Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses. Plant Molecular Biology, 69, 91-105. Kalde M, Barth M, Somssich I E, Lippok B. 2003. Members of the Arabidopsis WRKY group III transcription factors are part of different plant defense signaling pathways. Molecular Plant-Microbe Interactions, 16, 295-305. Liu X Q, Bai X Q, Qian Q, Wang X J, Chen M S, Chu C C. 2005. OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1. Cell Research, 15, 593-603. Liu X Q, Bai X Q, Wang X J, Chu C C. 2007. OsWRKY71, a rice transcription factor, is involved in rice defense response. Journal of Plant Physiology, 164, 969-979. Maeo K, Hayashi S, Kojima-Suzuki H, Morikami A, Nakamura D. 2001. Role of conserved residues of the WRKY domain in the DNA-binding of tobacco WRKY family proteins. Bioscience Biotechnology and Biochemistry, 65, 2428-2436. Maleck K, Levine A, Eulgem T, Morgan A, Schmid J, Lawton K A, Dangl J L, Dietrich R A. 2000. The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nature Genetics, 26, 403-410. Pandey S P, Somssich I E. 2009. The role of WRKY transcription factors in plant immunity. Plant Physiology, 150, 1648-1655. Peng Y L, Shishiyama J. 1988. Temporal sequence of cytological events in rice leaves affected with Pyricularia oryzae. Canadian Journal of Botany, 166, 730-735. Qiu D Y, Xiao J, Ding X H, Xiong M, Cai M, Cao Y L, Li X H, Xu C G, Wang S P. 2007. OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylateand jasmonate-dependent signaling. Molecular Plant-Microbe Interactions, 20, 492-499. Qiu Y P, Yu D Q. 2009. Over-expression of the stress-induced OsWRKY45 enhances disease resistance and drought tolerance in Arabidopsis. Environmental and Experimental Botany, 65, 35-47. Ramamoorthy R, Jiang S Y, Kumar N, Venkatesh P N, Ramachandran S. 2008. A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments. Plant and Cell Physiology, 49, 865-879. Ricachenevsky F K, Sperotto R A, Menguer P K, Fett J P. 2010. Identification of Fe-excess-induced genes in rice shoots reveals a WRKY transcription factor responsive to Fe, drought and senescence. Molecular Biology Reports, 37, 3735-3745. Ross C A, Liu Y, Shen Q J. 2007. The WRKY gene family in rice (Oryza sativa). Journal of Integrative Plant Biology, 49, 827- 842. Ryu H S, Han M, Lee S K, Cho J I, Ryoo N, Heu S, Lee Y H, Bhoo S H, Wang G L, Hahn T R, et al. 2006. A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response. Plant Cell Reports, 25, 836- 847. Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, et al. 2002. Monitoring the expression profiles of 7 000 Arabidopsis genes under drought, cold and high-salinity stresses using a fulllength cDNA microarray. The Plant Journal, 31, 279-292. Shimono M, Sugano S, Nakayama A, Jiang C J, Ono K, Toki S, Takatsuji H. 2007. Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance. The Plant Cell, 19, 2064-2076. Shinozaki K, Yamaguchi-Shinozaki K. 2000. Molecular responses to dehydration and low temperature: differences and crosstalk between two stress signaling pathways. Current Opinion in Plant Biology, 3, 217-223. Song Y, Jing S J, Yu D Q. 2009. Overexpression of the stressinduced OsWRKY08 improves osmotic stress tolerance in Arabidopsis. Chinese Science Bulletin, 54, 4671-4678. Triezenberg S J. 1995. Structure and function of transcriptional activation domains, Current Opinion in Genetics & Development, 5, 190-196. Turck F, Zhou A, Somssich I E. 2004. Stimulus-dependent, promoter-specific binding of transcription factor WRKY1 to its native promoter and the defense-related gene PcPR1-1 in parsley. The Plant Cell, 16, 2573-2585. Turner J G, Ellis C, Devoto A. 2002. The jasmonates signal pathway. The Plant Cell, 14, S153-164. Ülker B, Somssich I E. 2004. WRKY transcription factors: from DNA binding towards biological function. Current Opinion in Plant Biology, 7, 491-498. Wang H H, Hao J J, Chen X J, Hao Z N, Wang X, Lou Y G, Peng Y L, Guo Z J. 2007. Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants. Plant Molecular Biology, 65, 799-815. Wang K L, Li H, Ecker J R. 2002. Ethylene biosynthesis and signaling networks. The Plant Cell, 14, S131-151. Wang Z B, Zuo S M, Li G, Chen X J, Chen Z X, Zhang Y F, Pan X B. 2009. Rapid identification technology of resistance to rice sheath blight in seedling stage. Acta Phytopathologica Sinica, 39, 174-182. (in Chinese) Wu K L, Guo Z J, Wang H H, Li J. 2005. The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Research, 12, 9-26. Yi S Y, Kim J H, Joung Y H, Lee S, Kim W T, Yu S H, Choi D. 2004. The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis. Plant Physiology, 136, 2862-2874. Yu D, Chen C, Chen Z. 2001. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. The Plant Cell, 13, 1527-1540. Zhang J Z. 2003. Overexpression analysis of plant transcription factors. Current Opinion in Plant Biology, 6, 430-440. Zhang Y J, Wang L J. 2005. The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evolutionary Biology, 5, 1. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
