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Journal of Integrative Agriculture  2021, Vol. 20 Issue (1): 168-177    DOI: 10.1016/S2095-3119(20)63414-1
Special Issue: 植物病理合辑Plant Protection—Plant Pathology ; 线虫合辑Nematology 植物抗病遗传合辑Plant Disease-resistance Genetics
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Identification and resistant characterization of legumes sources against Meloidogyne incognita #br#
Pornthip RUANPANUN1, Prakit SOMTA2
1 Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
2 Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
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Abstract  
Root-knot nematodes (RKNs; Meloidogyne spp.) are becoming a serious problem in legume production.  This study identified Vigna genotypes exhibiting resistance to M. incognita (RKN) and characterized the modes of the resistance to M. incognita.  In total, 279 accessions from 21 Vigna species were screened for resistance based on a galling index (GI) and an egg mass index (EI).  Seven accessions were highly resistant to RKN with GI≤25, namely JP74716 (V. mungo var. mungo; cultivated black gram), JP107881 (V. nepalensis), JP229392 (V. radiata var. sublobata; wild mungbean), AusTRCF118141 (V. unguiculata subsp. unguiculata; cultivated cowpea), AusTRCF306385 (V. unguiculata subsp. unguiculata), AusTRCF322090 (V. vexillata var. vexillata; wild zombi pea) and JP235929 (V. vexillata var. vexillata).  JP229392 and AusTRCF322090 were the most resistant accessions having EI values of 18.74 and 1.88, respectively.  Continuous culture of M. incognita on both JP229392 and AusTRCF322090 resulted in a weakness in pathogenic ability for this RKN.  The resistance in JP229392 and AusTRCF322090 to RKN appeared to be antibiosis that was associated with reduced nematode penetration, retardation of nematode development and impeding giant cell formation.  The Vigna germplasm resistance to RKN identified in this study could be utilized as gene sources for the development of RKN-resistant Vigna cultivars.
 
Keywords:  host-plant resistance        root-knot nematode        Vigna radiata        Vigna vexillata  
Received: 30 September 2019   Accepted:
Fund: This work was supported by the Thailand Research Fund, Thailand (MRG6080274).
Corresponding Authors:  Correspondence Prakit Somta, Tel: +66-34-351887, Fax: +66-34-351890, E-mail: agrpks@ku.ac.th   
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Pornthip RUANPANUN, Prakit SOMTA. 2021. Identification and resistant characterization of legumes sources against Meloidogyne incognita #br#. Journal of Integrative Agriculture, 20(1): 168-177.

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