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Journal of Integrative Agriculture  2020, Vol. 19 Issue (10): 2597-2601    DOI: 10.1016/S2095-3119(20)63217-8
Special Issue: 植物病理合辑Plant Protection—Plant Pathology 植物病毒合辑Plant Virus
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Decreasing detection frequency of MITE (MCLas-A) in the population of ‘Candidatus Liberibacter asiaticus’ recently collected in southern China
CUI Xue-jin, ZENG Chun-hua, LIU Ke-hong, TENG Cai-ling, ZHOU Chang-yong, WANG Xue-feng
National Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing 400712, P.R.China
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Abstract  
An active miniature inverted-repeat transposable element (MITE), MCLas-A, was previously identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease).  To explore the recent transposition status of MCLas-A, 389 ‘Ca. L. asiaticus’ strains collected from nine regions in China were amplified using a specific primer set and three representative ‘Ca. L. asiaticus’ strains were analyzed by next-generation sequencing (NGS) approach.  PCR and genomic analysis showed that the entire MCLas-A was only present in 1.80% (7/389) and the jumping-out type of the MITE was predominant (81.23%) in samples tested, suggesting high frequency transposition occurred in ‘Ca. L. asiaticus’ strains recently collected from China.  Biological roles of transposition of the active MITE remain to be determined.
Keywords:  Huanglongbing        ‘Candidatus Liberibacter asiaticus&rsquo       miniature inverted-repeat transposable element        MITE  
Received: 30 December 2019   Accepted:
Fund: This study was funded by the National Natural Science Foundation of China (31671992, 31871925), the National Key R&D Program of China (2018YFD0201500), and the Key R&D Program of Guangxi, China (Gui Ke AA18118046).
Corresponding Authors:  Correspondence WANG Xue-feng, Tel: +86-23-68349338, E-mail: wangxuefeng@cric.cn   
About author:  CUI Xue-jin, E-mail: cuixuejin@163.com;

Cite this article: 

CUI Xue-jin, ZENG Chun-hua, LIU Ke-hong, TENG Cai-ling, ZHOU Chang-yong, WANG Xue-feng. 2020. Decreasing detection frequency of MITE (MCLas-A) in the population of ‘Candidatus Liberibacter asiaticus’ recently collected in southern China. Journal of Integrative Agriculture, 19(10): 2597-2601.

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