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Journal of Integrative Agriculture  2020, Vol. 19 Issue (10): 2530-2548    DOI: 10.1016/S2095-3119(20)63269-5
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Aggregate-associated changes in nutrient properties, microbial community and functions in a greenhouse vegetable field based on an eight-year fertilization experiment of China
LUAN Hao-an1, 2, GAO Wei3, TANG Ji-wei1, LI Ruo-nan4, LI Ming-yue3, ZHANG Huai-zhi1, CHEN Xin-ping2, Dainius MASILIUNAS5, HUANG Shao-wen1
1 Institute of Agricultural Resources and Regional Planning/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Center for Resources, Environment and Food Security, China Agricultural University, Beijing 100193, P.R.China
3 Tianjin Institute of Agricultural Resources and Environment, Tianjin 300192, P.R.China
4 Institute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, P.R.China
5 Laboratory of Geo-information Science and Remote Sensing, Wageningen University, Wageningen 476700, The Netherlands
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Abstract  
Soil aggregation, microbial community, and functions (i.e., extracellular enzyme activities; EEAs) are critical factors affecting soil C dynamics and nutrient cycling.  We assessed soil aggregate distribution, stability, nutrients, and microbial characteristics within >2, 0.25–2, 0.053–0.25, and <0.053 mm aggregates, based on an eight-year field experiment in a greenhouse vegetable field in China.  The field experiment includes four treatments: 100% N fertilizer (CF), 50% substitution of N fertilizer with manure (M), straw (S), and manure plus straw (MS).  The amounts of nutrient (N, P2O5, and K2O) input were equal in each treatment.  Results showed higher values of mean weight diameter in organic-amended soils (M, MS, and S, 2.43–2.97) vs. CF-amended soils (1.99).  Relative to CF treatment, organic amendments had positive effects on nutrient (i.e., available N, P, and soil organic C (SOC)) conditions, microbial (e.g., bacterial and fungal) growth, and EEAs in the >0.053 mm aggregates, but not in the <0.053 mm aggregates.  The 0.25–0.053 mm aggregates exhibited better nutrient conditions and hydrolytic activity, while the <0.053 mm aggregates had poor nutrient conditions and higher oxidative activity among aggregates, per SOC, available N, available P, and a series of enzyme activities.  These results indicated that the 0.25–0.053 mm (<0.053 mm) aggregates provide suitable microhabitats for hydrolytic (oxidative) activity.  Interestingly, we found that hydrolytic and oxidative activities were mainly impacted by fertilization (58.5%, P<0.01) and aggregate fractions (50.5%, P<0.01), respectively.  The hydrolytic and oxidative activities were significantly (P<0.01) associated with nutrients (SOC and available N) and pH, electrical conductivity, respectively.  Furthermore, SOC, available N, and available P closely (P<0.05) affected microbial communities within >0.25, 0.25–0.053, and <0.053 mm aggregates, respectively.  These findings provide several insights into microbial characteristics within aggregates under different fertilization modes in the greenhouse vegetable production system in China.
Keywords:  fertilization        soil aggregate distribution        microbial characteristics
  
Received: 10 February 2020   Accepted:
Fund: The authors sincerely acknowledge the financial support provided by the earmarked fund for China Agriculture Research System (CARS-23-B02), the National Key Research and Development Program of China (2016YFD0201001), and the Key Research and Development Program of Shandong Province, China (2017CXGC0206).
Corresponding Authors:  Correspondence HUANG Shao-wen, Tel: +86-10-82108662, E-mail: huangshaowen @caas.cn; TANG Ji-wei, Tel: +86-10-82105027, E-mail: tang-jiwei@163.com   
About author:  LUAN Hao-an, E-mail: luanhaoan@163.com;

Cite this article: 

LUAN Hao-an, GAO Wei, TANG Ji-wei, LI Ruo-nan, LI Ming-yue, ZHANG Huai-zhi, CHEN Xin-ping, Dainius MASILIUNAS, HUANG Shao-wen. 2020. Aggregate-associated changes in nutrient properties, microbial community and functions in a greenhouse vegetable field based on an eight-year fertilization experiment of China. Journal of Integrative Agriculture, 19(10): 2530-2548.

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