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| PGC-1α differentially regulates the mRNA expression profiles of genes related to myofiber type specificity in chicken |
SHAN Yan-ju, JI Gai-ge, ZOU Jian-min, ZHANG Ming, TU Yun-jie, LIU Yi-fan, JU Xiao-jun, SHU Jing-ting |
Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225125, P.R.China |
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Abstract Previous studies on mammals showed that peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) played a prominent role in regulating muscle fiber type transition and composition. However, the role of PGC-1α in chicken muscle has seldom been explored. To investigate the effect of PGC-1α on chicken skeletal muscles in this study, the PGC-1α gene was overexpressed or silenced in chicken primary myoblasts by using lentivirus, and then the effects of the PGC-1α gene overexpression and knockdown on the mRNA expression profile of genes related to myofiber type specificity were examined during fiber formation. The results showed that overexpression of PGC-1α from proliferation to differentiation was accompanied by the up-regulated expression of Pax7, MyoD, and CnAα, which was significantly (P<0.01) increased after one day of transfection (1I). The enhancement of MyoG, MEF2c, and MyHC SM expression lagged, which was improved significantly (P<0.01) after four days of transfection (1I3D). Overexpression of PGC-1α decreased (P<0.01) the MyHC FWM expression after four days of transfection (1I3D), and it had no significant impact (P>0.05) on the expression of CnB1, NFATc3, and MyHC FRM during myofiber formation. The effective silence (P<0.01) of PGC-1α by lentivirus mediating short hairpin RNA (shRNA) was detected after four days of transfection (1I3D) in cultures, and the lack of its function in chicken primary myoblasts significantly (P<0.01) down-regulated the expression of Pax7, MyoD, CnAα, MyoG, MEF2c, and MyHC SM, significantly (P<0.01) up-regulated the expression of MyHC FWM, and had no significant impact (P>0.05) on the expression of CnB1, NFATc3, and MyHC FRM. These results indicated that the role of PGC-1α in regulating the fiber type specificity of chicken skeletal muscles might be similar to that in mammals, which interplayed with key genes related to myocyte differentiation and calcineurin signaling pathway.
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Received: 11 June 2019
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31301967), the Natural Science Foundation of Jiangsu Province, China (BK20161322), the projects of Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province (JQLAB-ZZ-201703), the Major Breeding Programs in Jiangsu Province, China (PZCZ201728), the earmarked fund for China Agriculture Research System (CARS-41), the Independent Scientific Foundation of Public Welfare Scientific Institutes in Jiangsu Province, China (BM2018026) and the Open Projects of Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs of China (CGB-201704). |
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Corresponding Authors:
Correspondence SHU Jing-ting, Tel: +86-514-85599076, E-mail: shujingting@163.com
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| About author:
SHAN Yan-ju, Tel: +86-514-85599076, E-mail: shanyanjusyj @163.com; |
Cite this article:
SHAN Yan-ju, JI Gai-ge, ZOU Jian-min, ZHANG Ming, TU Yun-jie, LIU Yi-fan, JU Xiao-jun, SHU Jing-ting.
2020.
PGC-1α differentially regulates the mRNA expression profiles of genes related to myofiber type specificity in chicken. Journal of Integrative Agriculture, 19(8): 2083-2094.
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