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Journal of Integrative Agriculture  2020, Vol. 19 Issue (8): 2064-2071    DOI: 10.1016/S2095-3119(19)62806-6
Special Issue: 植物病理合辑Plant Protection—Plant Pathology 植物病毒合辑Plant Virus
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Identification of a novel emaravirus infecting lilac through next-generation sequencing
WANG Ya-qin1*, SONG Yu1*, CAO Meng-ji2, CHENG Qi1, WU Jian-xiang1, HU Tao1
1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2 National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, P.R.China
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Abstract  
Emaraviruses are multipartite negative-sense single-stranded viruses that usually infect flowering plants.  Using unbiased next-generation sequencing (NGS) approach, a novel virus from lilac leaves with yellow mottle symptom was identified.  Five viral genomic RNA segments were detected and completely sequenced.  The molecular characteristics of the viral RNAs and proteins resemble those of members of the genus Emaravirus.  The virus was found to be mechanically transmissible to Nicotiana benthamiana. Sequence alignment and phylogenetic analyses of the virus support the proposal of creating a new species in the genus Emaravirus for which the name lilac chlorotic ringspot-associated virus (LiCRaV) is proposed. 
Keywords:  LiCRaV        lilac        Fimoviridae        Emaravirus  
Received: 03 June 2019   Accepted:
Fund: This research was funded by the Laboratory Technology Research Program, Zhejiang University, China (SJS201809).
Corresponding Authors:  Correspondence HU Tao, E-mail: taohu1989@zju.edu.cn    
About author:  * These authors contributed equally to this study.

Cite this article: 

WANG Ya-qin, SONG Yu, CAO Meng-ji, CHENG Qi, WU Jian-xiang, HU Tao. 2020. Identification of a novel emaravirus infecting lilac through next-generation sequencing. Journal of Integrative Agriculture, 19(8): 2064-2071.

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