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Journal of Integrative Agriculture  2018, Vol. 17 Issue (10): 2196-2203    DOI: 10.1016/S2095-3119(17)61865-3
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Rapid and convenient transformation of cotton (Gossypium hirsutum L.) using in planta shoot apex via glyphosate selection
GUO Wen-fang1, Kevin Yueju Wang2, WANG Nan1, LI Jun1, LI Gang-qiang1, LIU De-hu1 
 
1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Department of Natural Sciences, Northeastern State University, Broken Arrow, Oklahoma 74014, USA
 
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Abstract  Cotton plants are recalcitrant with regards to transformation and induced regeneration.  In the present study, 5-enolpyruvylshikimate-3-phosphate (EPSPS), a glyphosate resistant gene from the bacterium Agrobacterium sp. strain CP4, was introduced into an elite Bt transgenic cotton cultivar with a modified technique involving in planta Agrobacterium-mediated transformation of shoot apex.  Primary transformants were initially screened using a 0.26% glyphosate spray and subsequently by PCR analysis.  Five out of 4 000 transformants from T1 seeds were obtained resulting in an in planta transformation rate of 0.125%.  Four homozygous lines were produced by continuous self-fertilization and both PCR-based selection and glyphosate resistance.  Transgene insertion was analyzed by Southern blot analysis.  Gene transcription and protein expression levels in the transgenic cotton lines were further investigated by RT-PCR, Western blot, and ELISA methods.  Transgenic T3 plants were resistant to as much as 0.4% of glyphosate treatments in field trials.  Our results indicate that the cotton shoot apex transformation technique which is both tissue-culture and genotype-independent would enable the exploitation of transgene technology in different cotton cultivars.  Since this method does not require sterile conditions, the use of specialized growth media or the application of plant hormones, it can be conducted under the greenhouse condition.
 
Keywords:  shoot apex transformation        CP4-EPSPS        glyphosate        in planta        transgenic cotton  
Received: 27 October 2017   Accepted:
Fund: This work was supported by the National Biotechnology Development Plan, China (2016ZX08005-004).
Corresponding Authors:  Correspondence LIU De-hu, Tel: +86-10-82109865, Fax: +86-10-82106107, E-mail: liudehu2006@126.com   
About author:  GUO Wen-fang, E-mail: guowf87@163.com;

Cite this article: 

GUO Wen-fang, Kevin Yueju Wang, WANG Nan, LI Jun, LI Gang-qiang, LIU De-hu. 2018. Rapid and convenient transformation of cotton (Gossypium hirsutum L.) using in planta shoot apex via glyphosate selection. Journal of Integrative Agriculture, 17(10): 2196-2203.

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