|
|
|
Isolation and characterization of the secondary wall-related SND1 gene in hawthorn |
CHEN Ke-qin1, GUO Yun-na2, SONG Meng-ru2, DAI Hong-yan2, ZHANG Zhi-hong1 |
1 Group of Molecular Biology of Fruit Trees, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, P.R.China
2 Group of Fruit Germplasm Evaluation & Utilization, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, P.R.China |
|
|
Abstract Secondary wall-associated NAC domain protein1 (SND1) is a key regulator directly regulating the expression levels of MYB46 and MYB83 in the regulation network for secondary wall synthesis, especially in plant fibres. In this study, a SND1 gene was isolated from hawthorn (Crataegus pinnatifida) and named as CpSND1 because it has a conservative N-terminal DNA-binding domain with AtSND1. Arabidopsis plants overexpressing CpSND1 had similar phenotypes as plants overexpressing AtSND1, including inhibited growth, upward-curling leaves, sepal dysplasia and sterility. In addition, overexpressing CpSND1 in Arabidopsis also induced the expression of downstream genes, including lignin, cellulose and xylan biosynthesis genes as well as MYB genes. Our results provided functional information of CpSND1 for future genetic engineering in hawthorn.
|
Received: 12 September 2017
Accepted:
|
Fund: This work was supported by the grants from the National Natural Science Foundation of China (31170635). |
Corresponding Authors:
Correspondence DAI Hong-yan, E-mail: daihy1970@163.com; ZHANG Zhi-hong, E-mail: zhang_sau@163.com
|
Cite this article:
CHEN Ke-qin, GUO Yun-na, SONG Meng-ru, DAI Hong-yan, ZHANG Zhi-hong.
2018.
Isolation and characterization of the secondary wall-related SND1 gene in hawthorn. Journal of Integrative Agriculture, 17(09): 2007-2014.
|
Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M. 1997. Genes involved in organ separation in Arabidopsis: An analysis of the cup-shaped cotyledon mutant. The Plant Cell, 9, 841–857.
Dai H, Han G, Yan Y, Zhang F, Liu Z, Li X, Li W, Ma Y, Li H, Liu Y, Zhang Z. 2013. Transcript assembly and quantification by RNA-Seq reveals differentially expressed genes between soft-endocarp and hard-endocarp hawthorns. PLoS ONE, 8, e72910.
Ko J H, Jeon H W, Kim W C, Kim J Y, Han K H. 2014. The MYB46/MYB83-mediated transcriptional regulatory programme is a gatekeeper of secondary wall biosynthesis. Annals of Botany, 114, 1099–1107.
Kubo M, Udagawa M, Nishikubo N, Horiguchi G, Yamaguchi M, Ito J, Mimura T, Fukuda H, Demura T. 2005. Transcription switches for protoxylem and metaxylem vessel formation. Genes & Development, 19, 1855–1860.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25, 402–408.
McCarthy R L, Zhong R, Ye Z H. 2009. MYB83 is a direct target of SND1 and acts redundantly with MYB46 in the regulation of secondary cell wall biosynthesis in Arabidopsis. Plant and Cell Physiology, 50, 1950–1964.
Mitsuda N, Iwase A, Yamamoto H, Yoshida M, Seki M, Shinozaki K, Ohme-Takagi M. 2007. NAC transcription factors, NST1 and NST3, are key regulators of the formation of secondary walls in woody tissues of Arabidopsis. The Plant Cell, 19, 270–280.
Mitsuda N, Seki M, Shinozaki K, Ohme-Takagi M. 2005. The NAC transcription factors NST1 and NST2 of Arabidopsis regulate secondary wall thickenings and are required for anther dehiscence. The Plant Cell, 17, 2993–3006.
Ohashi-Ito K, Oda Y, Fukuda H. 2010. Arabidopsis VASCULAR-RELATED NAC-DOMAIN6 directly regulates the genes that govern programmed cell death and secondary wall formation during xylem differentiation. The Plant Cell, 22, 3461–3473.
Ohtani M, Nishikubo N, Xu B, Yamaguchi M, Mitsuda N, Goué N, Shi F, Ohme-Takagi M, Demura T. 2011. A NAC domain protein family contributing to the regulation of wood formation in poplar. The Plant Journal, 67, 499–512.
Raven P H, Evert R F, Eichhorn S E. 1999. Biology of Plants. 6th ed. W.H. Freeman and Company, New York.
Taylor-Teeples M, Lin L, de Lucas M. 2015. An Arabidopsis gene regulatory network for secondary cell wall synthesis. Nature, 517, 571–575.
Yamaguchi M, Kubo M, Fukuda H, Demura T. 2008. VASCULAR-RELATED NAC-DOMAIN7 is involved in the differentiation of all types of xylem vessels in Arabidopsis roots and shoots. The Plant Journal, 55, 652–664.
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T. 2011. VASCULAR-RELATED NAC-DOMAIN 7 directly regulates the expression of a broad range of genes for xylem vessel formation. The Plant Journal, 66, 579–590.
Zhao Y, Sun J, Xu P, Zhang R, Li L. 2014. WOOD-ASSOCIATED NAC TRANSCRIPTION FACTOR1B regulates cell wall thickening during fiber development in Populus species. Plant Physiology, 164, 765–776.
Zhong R, Demura T, Ye Z H. 2006. SND1, a NAC domain transcription factor, is a key regulator of secondary wall synthesis in fibers of Arabidopsis. The Plant Cell, 18, 3158–3170.
Zhong R, Lee C, Ye Z H. 2010a. Functional characterization of poplar wood-associated NAC domain transcription factors. Plant Physiology, 152, 1044–1055.
Zhong R, Lee C, Ye Z H. 2010b. Global analysis of direct targets of secondary wall NAC master switches in Arabidopsis. Molecular Plant, 3, 1087–1103.
Zhong R, Lee C, Zhou J, McCarthy R L, Ye Z H. 2008. A battery of transcription factors involved in the regulation of secondary cell wall biosynthesis in Arabidopsis. The Plant Cell, 20, 2763–2782.
Zhong R, Richardson E A, Ye Z H. 2007. The MYB46 transcription factor is a direct target of SND1 and regulates secondary wall biosynthesis in Arabidopsis. The Plant Cell, 19, 2776–2792.
Zhong R, Ye Z H. 2014. Secondary cell walls: Biosynthesis, patterned deposition and transcriptional regulation. Plant and Cell Physiology, 56, 195–214.
Zhong R, Ye Z H. 2015a. Secondary cell walls: Biosynthesis, patterned deposition and transcriptional regulation. Plant and Cell Physiology, 56, 195–214.
Zhong R, Ye Z H. 2015b. The Arabidopsis NAC transcription factor NST2 functions together with SND1 and NST1 to regulate secondary wall biosynthesis in fibers of in?orescence stems. Plant Signaling Behavior, 10, e989746.
Zhou J, Zhong R, Ye Z H. 2014. Arabidopsis NAC domain proteins, VND1 to VND5, are transcriptional regulators of secondary wall biosynthesis in vessels. PLoS ONE, 9, e105726.
|
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|