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| Rapid semi-quantification of triacylglycerols, phosphatidylcholines, and free fatty acids in the rice bran of one grain |
| Junko Nagamoto1*, Ai Sawazaki1*, Motonori Miyago1, Bungo Shirouchi1, Mitsukazu Sakata2, TONG Li-tao1, Toshihiro Kumamaru2, Masao Sato1 |
1 Laboratory of Nutrition Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan
2 Laboratory of Plant Genetic Resources, Institute of Genetic Resources, Kyushu University, Fukuoka 812-8581, Japan |
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Abstract We developed a microplate assay method for determining the contents of triacylglycerols (TAGs), phosphatidylcholines (PCs), and free fatty acids (FFAs) in the rice bran of one grain using enzymatic reactions. In this method, enzymes from commercially available kits were used. Optimum reaction conditions were established. It was found that Nonidet P-40 was the optimal among the three surfactants used (Triton X-100, Tween 40, and Nonidet P-40) when lipid was dissolved in a reaction solution. Using this method, it was possible to quantify TAGs, PCs, and FFAs in concentration ranges of 7–150, 5–70, and 8–200 mg L–1, respectively. Furthermore, when the TAG contents in the rice bran were measured, the values closely corresponded to those obtained by extracting from large amounts of rice bran. However, sufficient data on the PC and FFA contents in rice bran are not available for valid comparisons. Although this method can accurately quantify the TAG contents in the rice bran of one grain, the accuracy of the PC and FFA contents has not been verified. Hence, future study is necessary.
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Received: 27 July 2017
Accepted:
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| Fund: This work was supported by the Ministry of Agriculture, Forestry and Fisheries, MAFF, Tokyo, Japan (27001B). We would like to thank Editage (www.editage.jp) for the English language editing. |
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Corresponding Authors:
Correspondence SATO Masao, Tel/Fax: +81-92-6423004, E-mail: masaos@agr.kyushu-u.ac.jp
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| About author:
* These authors contributed equally to this study. |
Cite this article:
Junko Nagamoto, Ai Sawazaki, Motonori Miyago, Bungo Shirouchi, Mitsukazu Sakata, TONG Li-tao, Toshihiro Kumamaru, Masao Sato.
2018.
Rapid semi-quantification of triacylglycerols, phosphatidylcholines, and free fatty acids in the rice bran of one grain. Journal of Integrative Agriculture, 17(05): 977-981.
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