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| A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat |
| WANG Yi-xue1, XU Qiao-fang2, CHANG Xiao-ping2, HAO Chen-yang2, LI Run-zhi1, JING Rui-lian2 |
1 College of Agronomy, Shanxi Agricultural University, Taigu 030801, P.R.China
2 National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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Abstract Stress associated proteins (SAPs) are the A20/AN1 zinc-finger proteins which confer to abiotic stresses in plants. In this study, TaSAP7-B, including two AN1 domains, was isolated from B genome of wheat (Triticum aestivum L.). Sequencing analysis on TaSAP7-B illustrated one InDel (insertion-deletion) and one SNP (single nucleotide polymorphism) in the promoter region while no diversity was observed in the coding region. On the basis of SNP in the promoter region (–260 bp), a dCAPS (derived cleaved amplified polymorphic sequences) marker SNP-260 was developed for TaSAP7-B. Using a natural population consisting of 262 wheat accessions, significant associations were detected between the marker SNP-260 and agronomic traits, such as plant height (PH), peduncle length (PL), length of penultimate internode (LPI), number of spike per plant (NSP), and 1 000-grain weight (TGW). Two genotypes were identified using marker SNP-260 in the natural population. Among them, the genotypes possessing C allele exhibited a higher TGW and shorter PH than the T genotypes. Hence, base C was considered as the superior allele. The dCAPS marker of TaSAP7-B can be instrumental for marker-assisted selection for high grain size and short plant height.
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Received: 20 February 2017
Accepted:
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| Fund: This work was supported by the National Key Research and Development Program of China (2016YFD0100605) and the National Natural Science Foundation of China (31271720). |
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Corresponding Authors:
Correspondence LI Run-zhi, Tel: +86-354-6288373, E-mail: rli2001@hotmail.com; JING Rui-lian, Tel/Fax: +86-10-82105829, E-mail: jingruilian@caas.cn
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| About author: WANG Yi-xue, Mobile: +86-13466832889, E-mail: ciciwyx@163.com; |
Cite this article:
WANG Yi-xue, XU Qiao-fang, CHANG Xiao-ping, HAO Chen-yang, LI Run-zhi, JING Rui-lian.
2018.
A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat. Journal of Integrative Agriculture, 17(2): 276-284.
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