|
|
|
|
|
| Identification and cell wall analysis of interspecific hybrids between Oryza sativa and Oryza ridleyi |
| YI Chuan-deng, LI Wei, WANG De-rong, JIANG Wei, HU Da-bang, ZHOU Yong, LIANG Guo-hua, GU Ming-hong |
| Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.China |
|
|
|
|
Abstract Oryza ridleyi is an allotetraploid wild species with the HHJJ genome, and Oryza sativa is a diploid cultivated rice that has the AA genome. Although the wide hybrid between the two species is difficult to obtain, we overcome this difficulty by young embryo rescue. An obvious heterosis was primarily found for the plant height, tillering ability, vegetative vigor, etc. However, the hybrid panicle and culm traits were found to resemble that of the wild rice parent, O. ridleyi, for the long awns, exoteric purple stigma, grain shattering, dispersed panicles, and culm mechanical strength. Genomic in situ hybridization (GISH) analysis was subsequently performed on the mitotic metaphase chromosome of the root tips, and we determined that the hybrid is an allotriploid with 36 chromosomes and its genomic constitution is AHJ. Chemical analyses conducted on the culm of O. sativa, O. ridleyi, and their interspecific hybrids showed that major changes occurred in the xylose, glucose, and arabinose concentrations, which are correlated with the specific hemicellulose polymer and cellulose components that are important in the primary cell walls of green plants. Meanwhile, the culm anatomical analyses indicated that additional large vascular bundles and an extra sclerenchyma cell layer were found in O. ridleyi. Additionally, further thickening of the secondary cell walls of the cortical fiber sclerenchyma cells and the phloem companion cells was discovered in O. ridleyi and in the interspecific hybrids. These results imply that there may be a potential link between culm mechanical strength and culm anatomical structure.
|
|
Received: 19 August 2016
Accepted:
|
| Fund: This work was supported by the National Natural Science Foundation of China (31571624 and 31071382), the National Key Research and Development Program of China (2016YFD0102001-006), the National Basic Research (973) Program of China (2010CB125904-3, 2013CBA01405), the Key Natural Science Project in University of Jiangsu Province, China (15KJA210004), and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD). |
|
Corresponding Authors:
Correspondence YI Chuan-deng, E-mail: yicd1973@126.com
|
Cite this article:
YI Chuan-deng, LI Wei, WANG De-rong, JIANG Wei, HU Da-bang, ZHOU Yong, LIANG Guo-hua, GU Ming-hong .
2017.
Identification and cell wall analysis of interspecific hybrids between Oryza sativa and Oryza ridleyi. Journal of Integrative Agriculture, 16(08): 1676-1681.
|
| Aggarwal R K, Brar D S, Khush G S. 1997. Two new genomes in the Oryza complex identified on the basis of molecular divergence analysis using total genomic DNA hybridization. Molecular and General Genetics, 254, 1–12.Amante-Bordeos A, Sitch L A, Nelson R, Dalmacio R D, Oliva N P, Aswidinnoor H, Leung H. 1992. Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice, Oryza sativa. Theoretical and Applied Genetics, 84, 345–354.Appenzeller L, Doblin M, Barreiro R, Wang H, Niu X, Kollipara K, Carrigan L, Tomes D, Chapman M, Dhugga K S. 2004. Cellulose synthesis in maize: Isolation and expression analysis of the cellulose synthase (CesA) gene family. Cellulose, 11, 287–299.Brar D S, Khush G S. 1997. Alien introgression in rice. Plant Molecular Biology, 35, 35–47.Fu X, Lu Y, Liu X, Li J, Feng J. 2007. Cytological mechanisms of interspecific incrossability and hybrid sterility between Oryza sativa L. and O. alta Swallen. Chinese Science Bulletin, 52, 755–765.Ge S, Sang T, Lu B R, Hong D Y. 1999. Phylogeny of rice genomes with emphasis on origins of allotetraploid species. Proceedings of the National Academy of Sciences of the United States of America, 96, 14400–14405.Jena K K, Khush G S. 1984. Embryo rescue of interspecific hybrids and its scope in rice improvement. Rice Genetics Newsletter, 1, 133–134.Jena K K, Khush G S. 1990. Introgression of genes from Oryza officinalis Well ex Watt to cultivated rice, O. sativa L. Theoretical and Applied Genetics, 80, 737–745.Kang T J, Yang M S. 2004. Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants. BMC Biotechnology, 4, 20.Khush G S. 1997. Origin, dispersal, cultivation and variation of rice. Plant Molecular Biology, 35, 25–34.Konig A, Cockburn A, Crevel R W, Debruyne E, Grafstroem R, Hammerling U, Kimber I, Knudsen I, Kuiper H A, Peijnenburg A A, Penninks A H, Poulsen M, Schauzu M, Wal J M. 2004. Assessment of the safety of foods derived from genetically modified (GM) crops. Food and Chemical Toxicology, 42, 1047–1088.Li Y, Qian Q, Zhou Y, Yan M, Sun L, Zhang M, Fu Z, Wang Y, Han B, Pang X, Chen M, Li J. 2003. BRITTLE CULM1, which encodes a COBRA-like protein, affects the mechanical properties of rice plants. The Plant Cell, 15, 2020–2031.Lu F, Ammiraju J S, Sanyal A, Zhang S, Song R, Chen J, Li G, Sui Y, Song X, Cheng Z, de Oliveira A C, Bennetzen J L, Jackson S A, Wing R A, Chen M. 2009. Comparative sequence analysis of MONOCULM1-orthologous regions in 14 Oryza genomes. Proceedings of the National Academy of Sciences of the United States of America, 106, 2071–2076.Multani D S, Jena K K, Brar D S, delos Reyes B C, Angeles E R, Khush G S. 1994. Development of monosomic alien addition lines and introgression of genes from Oryza australiensis. Domin. to cultivated rice O. sativa L. Theoretical and Applied Genetics, 88, 102–109.Seralini G E, de Vendomois J S, Cellier D, Sultan C, Buiatti M, Gallagher L, Antoniou M, Dronamraju K R. 2009. How subchronic and chronic health effects can be neglected for GMOs, pesticides or chemicals? International Journal of Biological Sciences, 5, 438–443.Wu Y, Sun Y, Shen K, Sun S, Wang J, Jiang T, Cao S, Josiah S M, Pang J, Lin X, Liu B. 2015. Immediate genetic and epigenetic changes in F1 hybrids parented by species with divergent genomes in the rice genus (Oryza). PLOS ONE, 10, e0132911.Xiong G, Li R, Qian Q, Song X, Liu X, Yu Y, Zeng D, Wan J, Li J, Zhou Y. 2010. The rice dynamin-related protein DRP2B mediates membrane trafficking, and thereby plays a critical role in secondary cell wall cellulose biosynthesis. The Plant Journal, 64, 56–70.Yan H, Xiong Z, Min S, Hu H, Zhang Z, Tian S, Tang S. 1997. The transfer of brown planthopper resistance from Oryza eichingeri to O. sativa. Acta Genetica Sinica, 24, 424–431. (in Chinese)Yi C, Tang S, Zhou Y, Liang G, Gong Z, Gu M. 2008. Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization. Chinese Science Bulletin, 53, 2973–2980.Zhang B, Zhou Y. 2011. Rice brittleness mutants: A way to open the ‘black box’ of monocot cell wall biosynthesis. Journal of Integrative Plant Biology, 53, 136–142 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
