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| Molecular identification of the cytoplasmic male sterile source from Dongxiang wild rice (Oryza rufipogon Griff.) |
| YAN Song1*, ZHU Shan1, 2*, MAO Ling-hua3*, HUANG Ren-liang1, XIONG Hong-liang1, SHEN Lin-jun1, SHEN Xian-hua1 |
1 Rice National Engineering Laboratory (Nanchang)/Nanchang Sub-center, National Rice Improvement Center/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, P.R.China
2 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education/College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, P.R.China
3 Jiangxi Super-rice Research and Development Center, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, P.R.China |
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Abstract The use of cytoplasm male sterility (CMS) is crucial for three-line hybrid seed production. Two types of CMS have been discovered from Dongxiang wild rice, namely the wild abortion type CMS (CMS-WA) and the Dongxiang wild type CMS (CMS-DW). In this study, we show the molecular identification of the two types of CMS in Dongxiang wild rice. WA352, which conferred CMS-WA, was not detected in Dongxiang wild rice, implying Dongxiang wild rice does not carry the CMS-WA source. Further analysis of WA352 in DY1A, a CMS-DW line, by PCR amplification and sequencing, revealed two insertion-deletion polymorphisms occurred in CMS-DW compared to WA352 of CMS-WA. It was reported that WA352 was comprised of an unknown origin sequence and partial sequences of three open reading frames (ORFs), orf284, orf224 and orf288. The 42-bp insertion was located between the two segments of orf224 and orf288, which created a new chimeric ORF, orf216. This new ORF was also detected in CMS-HL. Based on the 9-bp deletion in orf284, a specific mitochondrial marker of DW-M1 was developed, which could be used to specifically distinguish the DW-type source. Moreover, semi-quantitative RT-PCR analysis preliminarily suggested that both orf216 and orf284 could be considered as candidates for CMS-DW. These findings present a preliminary understanding of CMS-DW at the molecular level.
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Received: 15 August 2016
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31460343) and the Key Program for Science and Technology of Jiangxi Province, China (20152ACF60015). |
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Corresponding Authors:
Correspondence SHEN Xian-hua, Tel: +86-791-87090661, E-mail: shen_xh20000913@126.com
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Cite this article:
YAN Song, ZHU Shan, MAO Ling-hua, HUANG Ren-liang, XIONG Hong-liang, SHEN Lin-jun, SHEN.
2017.
Molecular identification of the cytoplasmic male sterile source from Dongxiang wild rice (Oryza rufipogon Griff.). Journal of Integrative Agriculture, 16(08): 1669-1675.
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