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Journal of Integrative Agriculture  2017, Vol. 16 Issue (06): 1347-1357    DOI: 10.1016/S2095-3119(16)61553-8
Special Issue: 线虫合辑Nematology
Plant Protection Advanced Online Publication | Current Issue | Archive | Adv Search |
Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato
JIN Na*, XUE Hui*, LI Wen-jing, WANG Xue-yan, LIU Qian, LIU Shu-sen, LIU Pei, ZHAO Jian-long, JIAN Heng

Department of Plant Pathology, China Agricultural University/Key Laboratory of Plant Pathology, Ministry of Agriculture, Beijing 100193, P.R.China

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Abstract  Streptomyces rubrogriseus HDZ-9-47, isolated from eggs of Meloidogyne spp., was evaluated as a potential biocontrol agent of Meloidogyne incognita under in vitro and protective field.  Microscopic observations showed that HDZ-9-47 parasitized eggs of M. incognita within 7 days.  In vitro, the culture filtrate of HDZ-9-47 caused 97.0% mortality of second-stage juveniles (J2s) of M. incognita and inhibited more than 50% egg hatching.  In the field, compared with the control, the root-knot index and J2s density in the treatment of drench the broth contained 1012 HDZ-9-47 spores were respectively reduced by 51.1 and 80.7% at 90 days post transplantation, which were better than that in other application doses and methods.  In addition, reduction rates of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofumigation was 87.1 and 91.0%, respectively, better than either of HDZ-9-47 or biofumigation used alone or fosthiazate treatment.  And tomato yield also increased by 16.1%.  Together, our results suggest that HDZ-9-47 could be an effective biocontrol agent of M. incognita, and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for M. incognita control.
Keywords:  Meloidogyne incognita      Streptomyces rubrogriseus      biocontrol, parasitism      nematicidal metabolites      control efficacy  
Received: 31 August 2016   Accepted:
Fund: 

We thank Dr. Liu Xingzhong (Institute of Microbiology, Chinese Academy of Science) for providing the HDZ-9-47 strain.  This research was financially supported by the National Basic Research Program of China (2013CB127501), the National Key Research and Development Program of China (2016YFD0201003) and the National Natural Science Foundation of China (31272021, 31571987).

Corresponding Authors:  JIAN Heng, Tel: +86-10-62731102, Fax: +86-10-62731102, E-mail: hengjian@cau.edu.cn   
About author:  JIN Na, E-mail: heidakingner@126.com

Cite this article: 

JIN Na, XUE Hui* LI Wen-jing, WANG Xue-yan, LIU Qian, LIU Shu-sen, LIU Pei, ZHAO Jian-long, JIAN Heng. 2017. Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato. Journal of Integrative Agriculture, 16(06): 1347-1357.

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