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Journal of Integrative Agriculture  2017, Vol. 16 Issue (04): 900-910    DOI: 10.1016/S2095-3119(16)61523-X
Animal Science · Veterinary Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Consuming fermented distillers’ dried grains with solubles (DDGS) feed reveals a shift in the faecal microbiota of growing and fattening pigs using 454 pyrosequencing
WANG Jin1, HAN Ye1, ZHAO Jin-zhao2, ZHOU Zhi-jiang1, FAN Huan3, 4

1 School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P.R.China

2 Tianjin Kuntai Environment & Energy Science and Technology Development Co., Ltd., Tianjin 300191, P.R.China

3 Tianjin Animal Science and Veterinary Research Institute, Tianjin 300381, P.R.China 4 Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin 300072, P.R.China

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Abstract  The objective of this study was to investigate pig fed by Bacillus coagulans-fermented distillers’ dried grains with solubles (DDGS) on the faecal microbial composition and diversity using 454 pyrosequencing.  Healthy crossbred (Duroc×Yorkshire×Landrace) growing and fattening pigs (n=48), with an average initial body weight of 65 kg, were divided into two groups (24 replicates per group; four pens per group; six pigs per pen), and given either DDGS feed as the control, or B. coagulans-fermented DDGS feed as the treatment.  Faecal samples were collected on day 0, 7, 14, 21, and 28.  DNA was extracted, and the V3–V6 region of the 16S rRNA gene was amplified.  The fermented DDGS feed affected the relative abundance of bacteria populations at the phylum, genus, and species levels.  At the genus level, the consumption of fermented DDGS feed led to higher relative abundances of faecal Prevotella, Lactobacillus, Clostridium, Bifidobacterium, Roseburia, and Bacillus, and lower relative abundances of faecal Escherichia, Ruminococcus, Dialister, unclassified Lachnospiraceae, unclassified Ruminococcaceae, and unclassified Enterobacteriaceae than in the control.  At the species level, the consumption of fermented DDGS feed led to higher relative abundances of faecal Prevotella sp., Lactobacillus johnsonii, Lactobacillus fermentum, Lactobacillus mucosae, Lactobacillus reuteri, Clostridium butyricum, Bifidobacterium sp., and Roseburia sp., and lower relative abundances of faecal Prevotella copri, Escherichia coli, Ruminococcus gnavus, Ruminococcus flavefaciens, and Dialister sp. than in the control.  Principal coordinates analysis indicated a distinct separation in the faecal microbial communities of pigs that were fed the fermented and unfermented DDGS feed.  Fermented DDGS feed significantly increased the average daily gain (ADG) of pigs, and significantly decreased the average daily feed intake (ADFI) of feed and feed/gain (F/G).  Thus, our results demonstrate a beneficial shift in the faecal microbiota of pigs consuming fermented DDGS feed, with potential applications in livestock production.
Keywords:   faecal microbiota      growing and fattening pigs      pyrosequencing      fermented DDGS feed  
Received: 04 March 2016   Accepted:
Fund: 

This work was by the Open Funding Project of the Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, China (20160315).

Corresponding Authors:  ZHOU Zhi-jiang, E-mail: zzj@tju.edu.cn; FAN Huan, E-mail: fanhuan13212107231@126.com    
About author:  WANG Jin, E-mail: choupiyuanyuan@163.com

Cite this article: 

WANG Jin, HAN Ye, ZHAO Jin-zhao, ZHOU Zhi-jiang, FAN Huan. 2017. Consuming fermented distillers’ dried grains with solubles (DDGS) feed reveals a shift in the faecal microbiota of growing and fattening pigs using 454 pyrosequencing. Journal of Integrative Agriculture, 16(04): 900-910.

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