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| Transgenic plum Prunus domestica L., clone C5 (cv. HoneySweet) for protection against sharka disease |
| Jaroslav Polák1, Jiban K Kundu1, Boris Krška2, Eva Beoni1, Petr Komínek1, Jitka Pívalová1, Jana Jarošová1 |
1 Division of Crop Protecting and Plant Health, Crop Research Institute, Prague 16106, Czech Republic
2 Horticultural Faculty Lednice, Mendel University Brno, Lednice 69144, Czech Republic |
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Abstract Sharka caused by Plum pox virus (PPV) is the most harmful disease of stone fruits in Europe and elsewhere in the world. There is no highly PPV resistant cultivar of plum. Biotech approach has led to the development of resistance through genetic engineering. In this study, we evaluated a transgenic plum Prunus domestica L., clone C5 (cv. HoneySweet), where the PPV resistance is based on RNA interference (RNAi). Resistance in C5 plums has been evaluated for PPV, Prune dwarf virus (PDV), and Apple chlorotic leaf spot virus (ACLSV) in a regulated field trial in the Czech Republic for 12 years (2002–2013). Even under high and permanent infection pressure introduced through graft inoculation of the viruses, PPV has been detected in C5 trees only in several leaves situated close to the point of inoculum grafting in the first nine years. Mild symptoms of PPV disappeared year by year. No PPV symptoms were observed in the last three years and results of ELISA detection were negative. Similar results were obtained, when RT-PCR was used for PPV detection. No natural infection of PPV by aphids was recorded in C5 plums, even if PPV infected plants were growing in the same experimental field in close vicinity. Co-infections of PPV with PDV and/or ACLSV had practically no influence on stability of resistance in C5 trees. Twelve years of field testing in the Czech Republic clearly demonstrated the high level of resistance of C5 either to PPV infection by graft inoculation or natural infection through aphid vectors.
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Received: 01 September 2016
Accepted:
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| Fund: Authors’ thank to Dr. R Scorza (USDA-ARS Appalachian Fruit Research Station in Kearneysville, WV, USA) for budwood of plum P. domestica, clone C5, to Dr. P Bartoš (Crop Research Institute, Prague, Czech Republic) for critically reading and correction of English, and Mrs. M Duchá?ová (Crop Research Institute, Prague, Czech Republic) for technical assistance. This work was supported by the Ministry of Agriculture of the Czech Republic (QI101A123, QJ1610186 and RO 0415). |
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Corresponding Authors:
Jaroslav Polák, Tel: +420-233022315, Fax: +420-233311592, E-mail: polak@vurv.cz
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Cite this article:
Jaroslav Polák, Jiban K Kundu, Boris Kr?ka, Eva Beoni, Petr Komínek, Jitka Pívalová, Jana Jaro?ová .
2017.
Transgenic plum Prunus domestica L., clone C5 (cv. HoneySweet) for protection against sharka disease. Journal of Integrative Agriculture, 16(03): 516-522.
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