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Journal of Integrative Agriculture  2016, Vol. 15 Issue (10): 2290-2298    DOI: 10.1016/S2095-3119(16)61415-6
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Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes
XIAO Wu-ming1, LUO Li-xin1, WANG Hui1, GUO Tao1, LIU Yong-zhu1, ZHOU Ji-yong1, ZHU Xiao-yuan2, YANG Qi-yun2, CHEN Zhi-qiang1
1 National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R.China
2 Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, P.R.China
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Abstract      Utilization of R (resistance) genes to develop resistant cultivars is an effective strategy to combat against rice blast disease. In this study, R genes Pi46 and Pita in a resistant accession H4 were introgressed into an elite restorer line Hang-Hui-179 (HH179) using the marker-assisted backcross breeding (MABB) procedure. As a result, three improved lines (e.g., R1791 carrying Pi46 alone, R1792 carrying Pita alone and R1793 carrying both Pi46 and Pita) were developed. The three improved lines had significant genetic similarities with the recurrent parent HH179. Thus, they and HH179 could be recognized as near isogenic lines (NILs). The resistance spectrum of the three improved lines, which was tested at seedling stage, reached 91.1, 64.7 and 97.1%, respectively. This was markedly broader than that of HH179 (23.5%). Interestingly, R1793 showed resistance to panicle blast but neither R1791 nor R1792 exhibited resistance at two natural blast nurseries. The results implied that the stacking of Pi46 and Pita resulted in enhanced resistance, which was unachievable by either R gene alone. Further comparison indicated that the three improved lines were similar to HH179 in multiple agronomic traits; including plant height, tillers per plant, panicle length, spikelet fertility, and 1 000-grain weight. Thus, the three improved lines with different R genes can be used as new sources of resistance for developing variety. There is a complementary effect between the two R genes Pi46 and Pita.
Keywords:  rice blast        resistance gene        improvement        marker-assisted selection  
Received: 30 December 2015   Accepted:
Fund: 

This research was supported by the grant from the Science and Technology Planning Project of Guangdong Province, China (2015A020209142) and by the earmarked fund for the Modern Agro-industry Technology Research System, China (CARS-01-12).

About author:  XIAO Wu-ming, E-mail: heredity24@126.com;

Cite this article: 

XIAO Wu-ming, LUO Li-xin, WANG Hui, GUO Tao, LIU Yong-zhu, ZHOU Ji-yong, ZHU Xiao-yuan, YANG Qi-yun, CHEN Zhi-qiang. 2016. Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes. Journal of Integrative Agriculture, 15(10): 2290-2298.

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