|
|
|
|
|
| Subcellular Cd accumulation characteristic in root cell wall of rice cultivars with different sensitivities to Cd stress in soil |
| LIU Bin1, CHEN Li2, CHEN Shi-bao1, LI Ning1, ZHENG Han1, JIN Ke1, PANG Huan-cheng1, MA Yi-bing1 |
1 Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Institute of Plant Protection and Environmental Protection, Beijing Academy of Agricultural and Forestry Science, Beijing 100097, P.R.China |
|
|
|
|
Abstract The variations of grain cadmiun (Cd) concentrations, translocation factors (TFs) of Cd from roots to shoots/grains of six rice cultivars, characterized with different Cd-sensitivities in polluted soil were studied, the selected rice cultivars were Xiangzao 17 (R1), Jiayu 211 (R2), Xiangzao 42 (R3), Zhuliangyou 312 (R4), Zhuliangyou 611 (R5), and Jinyou 463 (R6), respectively. The Cd subcellular distribution and Cd binding characteristics on subcellular fractions of rice root cell wall (CW) were further investigated. The results showed that the rice grain Cd contents varied significantly, with a maximum variation of 47.0% among the cultivars, the largest grain Cd content was observed with cultivar R1 (Cd-sensitivity cultivar) and the smallest with R5 (Cd-tolerance cultivar). The translocation factors of Cd from roots to shoots (TFshoot) and roots to grains (TFgrain) varied greatly among the cultivars. In general, the TFgrain of the cultivars followed the order of R1>R2>R3>R4> R6-R5. The Cd concentration (mg kg–1 FW) in the fraction of root CW, the fraction of cell wall removing pectin (CW-P) and the fraction of cell wall removing pectin and hemicellulose (CW-P-HC) of the cultivars generally followed the order of CW-P>CW>CW-P-HC; the ratios of Cd concentration (mg kg–1 FW) in the fraction of CW-P to that of CW were mostly more than 1.10, while the ratios of Cd concentration in the fraction of CW-P-HC to that of CW were mostly less than 0.60, indicating that Cd was mainly stored in the hemicellulose of the root CW. The ratios of Cd of CW-P-HC to CW generally followed the descending order of R1~R2>R3>R4>R5~R6 for the cultivars, which implied that hemicellulose is probably the main subcellular pool for transferring Cd into rice grain, and it restrains the translocation of Cd from shoot to the grain, especially for the Cd-tolerance cultivars (R5 and R6), the compartmentation of more Cd in hemicellulose in root CW is probably one of the main mechanisms for Cd tolerance of rice cultivars.
|
|
Received: 20 July 2015
Accepted:
|
| Fund: This research was financially supported by the National Natural Science Foundation of China (41271490, 21077131) and the National Key Research and Development Program of China (2016YFD0800707). |
|
Corresponding Authors:
CHEN Shi-bao, Tel: +86-10-82106722, E-mail: chenshibao@caas.cn
|
| About author: LIU Bin, E-mail: 1051175517@qq.com; |
Cite this article:
LIU Bin, CHEN Li, CHEN Shi-bao, LI Ning, ZHENG Han, JIN Ke, PANG Huan-cheng, MA Yi-bing.
2016.
Subcellular Cd accumulation characteristic in root cell wall of rice cultivars with different sensitivities to Cd stress in soil. Journal of Integrative Agriculture, 15(9): 2114-2122.
|
| Chen S B, Sun C, Wei W, Lin L, Wang M. 2012. Difference in cell wall components of roots and its effect on the transfer factor of Zn by plant species. China Environmental Science, 32, 1309–1313. Clemens S, Aarts M G M, Thomine S, Verbruggen N. 2013. Plant science: The key to preventing slow cadmium poisoning. Trends in Plant Science, 18, 92–99.Douchiche O, Chaïbi W, Morvan C. 2012. Cadmium tolerance and accumulation characteristics of mature flax, cv. hermes: Contribution of the basal stem compared to the root. Journal of Hazardous Materials, 235–236, 101–107.Grant C A, Clarke J M, Duguid S, Chaney R L. 2008. Selection and breeding of plant cultivars to minimize cadmium accumulation. Science of the Total Environment, 390, 301–310.He J Y, Ren Y F, Wang F J, Pan X B, Zhu C, Jiang D A. 2008. Characterization of cadmium uptake and translocation in a cadmium-sensitive mutant of rice (Oryza sativa L. ssp. japonica). Archives of Environmental Contamination and Toxicology, 57, 299–306.Horiguchi H, Oguma E, Sasaki S. 2013. Age-relevant renal effects of cadmium exposure through consumption of home-harvested rice in female Japanese farmers. Environment International, 56, 1–9.Hu G H, Huang S H, Chen H, Wang F. 2010. Binding of four heavy metals to hemicelluloses from rice bran. Food Research International, 43, 203–206. Jiang X Y, Wang C H. 2007. Cadmium distribution and its effects on molybdate-containing hydroxylases in Phragmites australis. Aquatic Botany, 86, 353–360. Khan S, Brian J R, Li G, Zhu Y G. 2014. Application of biochar to soil reduces cancer risk via rice consumption: A case study in Miaoqian village, longyan, China. Environment International, 68, 154–161.Kirkham M B. 2006. Cadmium in plants on polluted soils: Effects of soil factors, hyperaccumulation, and amendments. Geoderma, 137, 19–32.Li Y H, Qian Q, Zhou Y H. 2003. Brittle CULM1, which encodes a COBRA-like protein, affects the mechanical properties of rice plants. The Plant Cell, 15, 2020–2031.Li Z, Li L, Chen G P. 2005. Bioavailability of Cd in a soil-rice system in China: Soil type versus genotype effects. Plant and Soil, 271, 165–173.Liu J G, Qu P, Zhang W, Dong Y, Wang M X. 2014. Variations among rice cultivars in subcellular distribution of Cd: The relationship between translocation and grain accumulation. Environmental and Experimental Botany, 107, 25–31.Liu J, Qian M, Cai G, Zhu Q, Wong M. 2007. Variations between rice cultivars in root secretion of organic acids and the relationship with plant cadmium uptake. Environmental Geochemistry and Health, 29, 189–195.Lu R K. 2000. Soil and Agro-chemical Analysis Methods. Agricultural Science and Technology Press, Beijing, China.(in Chinese)MacFarlane G R, Burchett M D. 2000. Cellular distribution of copper, lead and zinc in the grey mangrove. Aquatic Botany, 68, 45–59.Ramos I, Esteban E, Lucena J J. 2002. Cadmium uptake and subcellular distribution in plants of Lactuca sp. Cd/Mn interaction. Plant Science, 162, 761–767.Sebastian A, Prasad M N V. 2014. Cadmium minimization in rice. A review. Agronomy for Sustainable Development, 34, 155–173.Song W E, Chen S B. 2014. The toxicity thresholds (ECx) of cadmium to rice cultivars as determined by root elongation tests in soils and its predicted models. Scientia Agricultura Sinica, 47, 3434–3443. (in Chinese) Tarek M, Hana G, Shehata S. 2015. Bioaccumulation and translocation of heavy metals by Plantago major grown in contaminated soils under the effect of traffic pollution. Ecological Indicators, 48, 244–251. Uraguchi S, Fujiwara T. 2012. Cadmium transport and tolerance in rice: Perspectives for reducing grain cadmium accumulation. Rice, 5, 1–8.Uraguchi S, Mori S, Kuramata M, Kawasaki A, Arao T, Ishikawa S. 2009. Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice. Journal of Experimental Botany, 60, 2677–2688.Wang M E, Chen W P, Peng C. 2016. Risk assessment of Cd polluted paddy soils in the industrial and township areas in Hunan, Southern China. Chemosphere, 144, 346–351.Wei S H, Li Y M, Zhan J, Wang S S, Zhu J G. 2012. Tolerant mechanisms of Rorippa globosa (Turcz.) thell. hyperaccumulating Cd explored from root morphology. Bioresource Technology, 118, 455–459.Yu H, Wang J L, Fang W. 2006. Cadmium accumulation in different rice cultivars and screening for pollution-safe cultivars of rice. Science of the Total Environment, 370, 302–309. Yu H, Xiang Z X, Zhu Y, Wang J L, Yang Z J. 2012. Subcellular and molecular distribution of cadmium in two rice genotypes with different levels of cadmium accumulation. Journal of Plant Nutrition, 35, 71–84.Zhang J, Duan G L. 2009. Genotypic difference in arsenic and cadmium accumulation by rice seedlings grown in hydroponics. Journal of Plant Nutrition, 31, 2168–2182.Zhang X H, Lin A J, Gao Y L, Reid J, Zhu Y G. 2009. Arbuscular mycorrhizal colonisation increases copper binding capacity of root cell walls of Oryza sativa L. and reduces copper uptake. Soil Biology & Biochemistry, 41, 930–935. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
