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| A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks |
| BAI Miao1*, CHEN Wen-ting1*, XIE Bing-yan2, YANG Guo-shun1 |
1 College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, P.R.China
2 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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Abstract Cucumber mosaic virus (CMV) can infect a wide range of host species. For the lacking of CMV resistant varieties of tomato, RNA interference (RNAi) can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA (ihpRNA) plant expression vectors aimed at five genes of CMV have been constructed. Transgenic tomatoes were obtained by Agrobacterium tumefaciens-mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism (GMO) biosafety.
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Received: 12 November 2015
Accepted:
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| Fund: This work was supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (20134320120013) and the Natural Science Foundation of Hunan Province of China (14JJ3095). |
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Corresponding Authors:
YANG Guo-shun, Tel: +86-731-84618767, Fax: +86-731-84618171, E-mail: guoshunyang@foxmail.com
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| About author: BAI Miao, Mobile: +86-18374879885, E-mail: baimiao1984@qq.com; |
Cite this article:
BAI Miao, CHEN Wen-ting, XIE Bing-yan, YANG Guo-shun.
2016.
A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks. Journal of Integrative Agriculture, 15(9): 2040-2048.
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