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Journal of Integrative Agriculture  2018, Vol. 17 Issue (09): 2024-2030    DOI: 10.1016/S2095-3119(18)61962-8
Special Issue: 植物细菌真菌合辑Plant Bacteria/Fungus
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Germination and appressorium formation of Pyricularia oryzae Cavara can be inhibited by reduced concentration of Blasin®Flowable with carbon dioxide microbubbles
Tamaki Masahiko1, Kobayashi Fumiyuki2, Suehiro Keisuke1, Ohsato Shuichi1, Sato Michio
1 School of Agriculture, Meiji University, Kawasaki 214-8571, Japan
2 Faculty of Applied Life Science, Nippon Veterinary and Life Science University, Musashino 180-8602, Japan
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Abstract  
We investigated the possibility to reduce the usage of Blasin®Flowable (BF), a disinfectant inhibiting the germination and appressorium formation of Pyricularia oryzae Cavara conidia, by using carbon dioxide microbubbles (CO2MB).  Germination was significantly inhibited by 10 000-fold diluted BF solution containing CO2MB generated by the decompression-type generator compared to CO2 millibubbles (CO2MMB) and CO2MB generated by the gas-water circulating-type generator.  Appressorium formation in the 10 000-fold diluted BF solution containing both CO2MBs was less than that in CO2MMB.  Scanning electron microscopy showed wrinkles and dents on the surface of conidia treated with 5 000-fold diluted BF solution containing both CO2MBs.  Via transmission electron microscopy, we observed the expansion of the vacuole and the intracellular space and bloated or absent lipid granules in the conidia treated with BF solution containing both CO2MBs.  Our results show that inhibition of the conidium germination and appressorium formation of P. oryzae Cavara by 10 000-fold diluted BF solution could be achieved by using the decompression-type CO2MB.
 
Keywords:  appressorium formation        Blasin®Flowable       germination        Pyricularia oryzae        carbon dioxide microbubble  
Received: 31 July 2017   Accepted:
Corresponding Authors:  Correspondence Tamaki Masahiko, Tel/Fax: +81-44-980-5276,E-mail: mtamaki@meiji.ac.jp   

Cite this article: 

Tamaki Masahiko, Kobayashi Fumiyuki, Suehiro Keisuke, Ohsato Shuichi, Sato Michio. 2018. Germination and appressorium formation of Pyricularia oryzae Cavara can be inhibited by reduced concentration of Blasin®Flowable with carbon dioxide microbubbles. Journal of Integrative Agriculture, 17(09): 2024-2030.

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