Please wait a minute...
Journal of Integrative Agriculture  2016, Vol. 15 Issue (11): 2461-2468    DOI: 10.1016/S2095-3119(16)61379-5
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Identification of a new stripe rust resistance gene in Chinese winter wheat Zhongmai 175
LU Jia-ling1, CHEN Can1, 2, LIU Peng1, HE Zhong-hu1, 3, XIA Xian-chun1
1 National Wheat Improvement Center/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, P.R.China
2 College of Agronomy, Anhui Agricultural University, Hefei 230036, P.R.China
3 International Maize and Wheat Improvement Center (CIMMYT) China Office, Beijing 100081, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract  Stripe rust is a serious foliar disease posing a grave threat to wheat production worldwide.  The most economical and environmentally friendly way to control this disease is to breed and deploy resistant cultivars.  Zhongmai 175 is an elite winter wheat cultivar conferring resistance to a broad spectrum of Puccinia striiformis f. sp. tritici (Pst) races.  To identify the resistance gene in the cultivar, genetic analysis was conducted using the parents, F1, F2 and F2:3 populations derived from the cross of Lunxuan 987/Zhongmai 175.  Segregations in the F2 and F2:3 populations indicated a single dominant gene conferring resistance to stripe rust in Zhongmai 175, temporarily designated YrZM175.  Bulked segregant analysis (BSA) with wheat iSelect 90K SNP array determined a preliminary location of YrZM175.  Subsequently, YrZM175 was mapped on chromosome 2AS using simple sequence repeats (SSR), expressed sequence tags (EST) and newly-developed kompetitive allele specific PCR (KASP) markers, being flanked by Xgwm636 and Xwmc382 at genetic distances of 4.9 and 8.1 cM, respectively.  Comparison of reaction patterns of YrZM175 on 23 Pst races or isolates and pedigree analysis with other genes on chromosome 2AS suggested that it is likely to be a new gene for resistance to stripe rust.  The resistance gene and linked molecular markers will be useful in wheat breeding targeting for the improvement of stripe rust resistance.
Keywords:  molecular markers        Puccinia striiformis        SSR        SNP        Triticum aestivum  
Received: 06 November 2015   Accepted:
Fund: 

This study was supported by the National Basic Research Program of China (2013CB127700), the National Natural Science Foundation of China (31261140370), the International Collaboration Projects from the Ministry of Science and Technology, China (2013DFG30530), and the China Agriculture Research System (CARS-3-1-3).

Corresponding Authors:  XIA Xian-chun, E-mail: xiaxianchun@caas.cn   
About author:  LU Jia-ling, E-mail: lujialing92@126.com

Cite this article: 

LU Jia-ling, CHEN Can, LIU Peng, HE Zhong-hu, XIA Xian-chun. 2016. Identification of a new stripe rust resistance gene in Chinese winter wheat Zhongmai 175. Journal of Integrative Agriculture, 15(11): 2461-2468.

Bariana H S, McIntosh R A. 1993. Cytogenetic studies in wheat XV Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome, 36, 476–482.

Basnet B R, Singh R P, Ibrahim A M, Herrera-Foessel S A, Huerta-Espino J, Lan C X, Rudd J C. 2013. Characterization of Yr54 and other genes associated with adult plant resistance to yellow rust and leaf rust in common wheat Quaiu3. Molecular Breeding, 33, 385–399.

Cavanagh C R, Chao S M, Wang S C, Huang B E, Stephen S, Kiani S, Forrest K, Saintenac C, Brown-Guedira G L, Akhunova A, See D, Bai G, Pumphrey M, Tomar L, Wong D, Kong S, Reynolds M, Silva M L, Bockelman H, Talbert L, et al. 2013. Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proceedings of the National Academy of Sciences of the United States of America, 110, 8057–8062.

Chen W Q, Kang Z S, Ma Z H, Xu S C, Jin S L, Jiang Y Y. 2013. Integrated management of wheat stripe rust caused by Puccinia striiformis f. sp. tritici in China. Scientia Agricultura Sinica, 46, 4254–4262. (in Chinese)

Chen X M. 2005. Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat. Canadian Journal of Plant Pathology, 27, 314–337.

Chen X M. 2013. High-temperature adult-plant resistance, key for sustainable control of stripe rust. American Journal of Plant Sciences, 4, 608–627.

Chen X M. 2014. Integration of cultivar resistance and fungicide application for control of wheat stripe rust. Canadian Journal of Plant Pathology, 36, 311–326.

Chen X M, He Z H, Wang D S. 2009a. The National registered new wheat variety: Zhongmai 175. China Seed Industry, 7, 69. (in Chinese)

Chen X M, He Z H, Wang D S, Zhuang Q S, Zhang Y H, Zhang Y, Zhang Y, Xia X C. 2009b. Developing high yielding wheat varieties from core parent “Jing 411”. Crops, 4, 1–5. (in Chinese)

Cheng P, Xu L S, Wang M N, See D R, Chen X M. 2014. Molecular mapping of genes Yr64 and Yr65 for stripe rust resistance in hexaploid derivatives of durum wheat accessions PI331260 and PI480016. Theoretical and Applied Genetics, 127, 2267–2277.

Delannay X, McLaren G, Ribaut J M. 2012. Fostering molecular breeding in developing countries. Molecular Breeding, 29, 857–873.

Dragan P, Kopahnke D, Steffenson B J, Förster J, König J, Kilian B, Plieske J, Durstewitz G, Korzun V, Kraemer I, Habekuss A, Johnston P, Pickering R, Ordon F. 2012. Genetic fine mapping of a novel leaf rust resistance gene and a barley yellow dwarf virus tolerance (BYDV) introgressed from Hordeum bulbosum by the use of the 9K iSelect chip. In: Zhang G P, Li C D, Liu X, eds., Advance in Barley Sciences. Springer and Zhejiang University Press, Germany and China. pp. 269–284.

Hao Y F, Chen Z B, Wang Y Y, Bland D, Buck J, Brown-Guedira G, Johnson J. 2011. Characterization of a major QTL for adult plant resistance to stripe rust in US soft red winter wheat. Theoretical and Applied Genetics, 123, 1401–1411.

He C L, Holme J, Anthony J. 2014. SNP genotyping: The KASP assay. Methods in Molecular Biology, 1145, 75–86.

Helguera M, Khan I A, Kolmer J, Lijavetzky D, Zhong Q L, Dubcovsky J. 2003. PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Science, 43, 1839–1847.

Hyten D L, Smith J R, Frederick R D, Tucker M L, Song Q J, Cregan P B. 2009. Bulked segregant analysis using the GoldenGate assay to locate the Rpp3 locus that confers resistance to soybean rust in soybean. Crop Science, 49, 265–271.

Jighly A, Oyiga B C, Makdis F, Nazari K, Youssef O, Tadesse W, Abdalla O, Ogbonnaya F C. 2015. Genome-wide DArT and SNP scan for QTL associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in elite ICARDA wheat (Triticum aestivum L.) germplasm. Theoretical and Applied Genetics, 128, 1277–1295.

Kosambi D D. 1943. The estimation of map distances from recombination values. Annals of Eugenics, 12, 172–175.

Li C L, Jin F, Zhang Y Y, Zhao P, Wang Z H, Bai G H. 2014. Quantitative trait loci for stripe rust in the wheat RIL population Ning 7840×Clark. Journal of Triticeae Crops, 34, 157–163. (in Chinese)

Li J B, Lan M Q, Chen M Q, Bi Y Q, Yang J L, Chen X D, Li Y Q, Liu L, Liu T G, Zhu Y Y, Li C Y. 2013. Population analysis with race-specific-markers of Puccinia striiformis f. sp. tritici from two counties of Yunnan Province. Acta Phytopathological Sinica, 43, 643–646. (in Chinese)

Li Y, Niu Y C. 2006. Genetic analysis of stripe rust in wheat variety C591. Plant Protection, 32, 39–41. (in Chinese)

Lincoln S E, Daly M J, Lander E S. 1993. Constructing genetic maps with MAPMAKER/EXP 3.0. In: Whitehead Institute Technical Report. 3rd ed. Cambridge, Whitehead Institute, MA.

Line R F. 2002. Stripe rust of wheat and barley in North America: a retrospective historical review. Annual Review of Phytopathology, 40, 75–118.

Liu L, Lan M Q, Yang J, Zhang B, Li J, Li C Y. 2013a. Analysis population structure of Puccinia striiformis f. sp. tritici using race-specific-marker in Yunnan during 2009–2010. Acta Phytophylacica Sinica, 40, 89–90. (in Chinese)

Liu L, Lan M Q, Zhang B, Yang J, Zhao J, Gu Z L, Zhang P H, Li C Y. 2013b. Analysis physiological strain of Puccinia striifomis f. sp. tritici in Yunnan during 2010–2011 by its race-specific-marker. Journal of Yunnan Agricultural University (Natural Science), 38, 625–630. (in Chinese)

Liu R H, Meng J L. 2003. MapDraw: A Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas, 25, 317–321. (in Chinese)

Liu S B, Yang X P, Zhang D D, Bai G H, Chao S M, Bockus W. 2014. Genome?wide association analysis identified SNPs closely linked to a gene resistant to soil-borne wheat mosaic virus. Theoretical and Applied Genetics, 127, 1039–1047.

Lu Y Q, Wu X Y, Yao M M, Zhang J P, Liu W H, Yang X M, Li X Q, Du J, Gao A N, Li L H. 2015. Genetic mapping of a putative Agropyron cristatum-derived powdery mildew resistance gene by a combination of bulked segregant analysis and single nucleotide polymorphism array. Molecular Breeding, 35, 1–13.

Lu Y, Wang M N, Chen X M, Deven S, Chao S M, Jing J X. 2014. Mapping of Yr62 and a small?effect QTL for high?temperature adult?plant resistance to stripe rust in spring wheat PI 192252. Theoretical and Applied Genetics, 127, 1449–1459.

McIntosh R A, Dubcovsky J, Rogers W J, Morris C, Appels R, Xia X C. 2014. Catalogue of gene symbols for wheat: 2013–2014 Supplement. [2015-09-06]. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement 2013.pdf

Murray R A, Thompson W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321–4325.

Naruoka Y, Garland-Campbell K A, Carter A H. 2015. Genome-wide association mapping for stripe rust (Puccinia striiformis f. sp. tritici) in US Pacific Northwest winter wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 128, 1083–1101.

Ribaut J M, de Vicente M C, Delannay X. 2010. Molecular breeding in developing countries: Challenges and perspectives. Current Opinion in Plant Biology, 13, 1–6.

Robinson P, Ganske F. 2007. High speed FRET based SNP genotyping measurement on the PHERAstar. [2014-03-04]. http://www.bmglabtech.com/media/35297/160-poster-snp-fret-1049105.pdf

Rosewarne G M, Herrera-Foessel S A, Singh R P, Huerta-Espino J, Lan C X, He Z H. 2013. Quantitative trait loci of stripe rust resistance in wheat. Theoretical and Applied Genetics, 126, 2427–2449.

Semagn K, Babu R, Hearne S, Olsen M. 2014. Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): Overview of the technology and its application in crop improvement. Molecular Breeding, 33, 1–14.

Sukumaran S, Dreisigacker S, Lopes M, Chavez P, Reynolds M P. 2015. Genome-wide association study for grain yield and related traits in an elite spring wheat population grown in temperate irrigated environments. Theoretical and Applied Genetics, 128, 353–363.

Stubbs R W. 1985. Stripe Rust. In: Roelfs A P, Bushnell W R, eds., The Cereal Rusts. vol. II. Academic Press, Orlando, USA. pp. 61–101.

Wang S C, Wong D, Forrest K, Allen A, Chao S M, Huang B E, Maccaferri M, Salvi S, Milner S G, Cattivelli L, Mastrangelo A M, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, IWGSC (International Wheat Genome Sequencing Center), Lillemo M, Mather D, Appels R, et al. 2014. Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array. Plant Biotechnology Journal, 12, 787–796.

Wei J X, Geng H W, Zhang Y, Liu J D, Wen W E, Zhang Y, Xia X C, Chen X M, He Z H. 2015. Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL. Theoretical and Applied Genetics, 128, 2067–2076.

Wellings C R. 2011. Global status of stripe rust: A review of historical and current threats. Euphytica, 179, 129–141.

Xu L S, Wang M N, Cheng P, Kang Z S, Hulbert S H, Chen X M. 2013. Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat. Theoretical and Applied Genetics, 126, 523–533.

Yuan T. 1988. Physiological race of wheat stripe rust CYR29 was rising rapidly. Seeds Communication, 4, 43. (in Chinese)

Zhou X L, Han D J, Chen X M, Gou H L, Guo S J, Rong L, Wang Q L, Huang L L, Kang Z S. 2014a. Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34. Theoretical and Applied Genetics, 127, 2349–2358.

Zhou X L, Wang M N, Chen X M, Lu Y, Kang Z S, Jing J X. 2014b. Identification of Yr59 conferring high-temperature adult-plant resistance to stripe rust in wheat germplasm PI 178759. Theoretical and Applied Genetics, 127, 935–945.
[1] WU Bang-bang, SHI Meng-meng, Mohammad POURKHEIRANDISH, ZHAO Qi, WANG Ying, YANG Chen-kang, QIAO Ling, ZHAO Jia-jia, YAN Su-xian, ZHENG Xing-wei, ZHENG Jun. Allele mining of wheat ABA receptor at TaPYL4 suggests neo-functionalization among the wheat homoeologs[J]. >Journal of Integrative Agriculture, 2022, 21(8): 2183-2196.
[2] LIU Lei, WANG Heng-bo, LI Yi-han, CHEN Shu-qi, WU Ming-xing, DOU Mei-jie, QI Yi-yin, FANG Jing-ping, ZHANG Ji-sen. Genome-wide development of interspecific microsatellite markers for Saccharum officinarum and Saccharum spontaneum[J]. >Journal of Integrative Agriculture, 2022, 21(11): 3230-3244.
[3] YANG Meng-jiao, WANG Cai-rong, Muhammad Adeel HASSAN, WU Yu-ying, XIA Xian-chun, SHI Shu-bing, XIAO Yong-gui, HE Zhong-hu. QTL mapping of seedling biomass and root traits under different nitrogen conditions in bread wheat (Triticum aestivum L.)[J]. >Journal of Integrative Agriculture, 2021, 20(5): 1180-1192.
[4] NIE Xing-hua, WANG Ze-hua, LIU Ning-wei, SONG Li, YAN Bo-qian, XING Yu, ZHANG Qing, FANG Ke-feng, ZHAO Yong-lian, CHEN Xin, WANG Guang-peng, QIN Ling, CAO Qing-qin. Fingerprinting 146 Chinese chestnut (Castanea mollissima Blume) accessions and selecting a core collection using SSR markers[J]. >Journal of Integrative Agriculture, 2021, 20(5): 1277-1286.
[5] DIAO Shu-qi, XU Zhi-ting, YE Shao-pan, HUANG Shu-wen, TENG Jin-yan, YUAN Xiao-long, CHEN Zan-mou, ZHANG Hao, LI Jia-qi, ZHANG Zhe. Exploring the genetic features and signatures of selection in South China indigenous pigs[J]. >Journal of Integrative Agriculture, 2021, 20(5): 1359-1371.
[6] HU Fu-chu, CHEN Zhe, WANG Xiang-he, WANG Jia-bao, FAN Hong-yan, QIN Yong-hua, ZHAO Jietang, HU Gui-bing. Construction of high-density SNP genetic maps and QTL mapping for dwarf-related traits in Litchi chinensis Sonn[J]. >Journal of Integrative Agriculture, 2021, 20(11): 2900-2913.
[7] REN Yun, CHEN Dan, LI Wen-jie, TAO Luo, YUAN Guo-qiang, CAO Ye, LI Xue-mei, DENG Qi-ming, WANG Shi-quan, ZHENG Ai-ping, ZHU Jun, LIU Huai-nian, WANG Ling-xia, LI Ping, LI Shuang-cheng . Genome-wide pedigree analysis of elite rice Shuhui 527 reveals key regions for breeding[J]. >Journal of Integrative Agriculture, 2021, 20(1): 35-45.
[8] CUI Hong-ying, ZHAO Zhang-wu. Structure and function of neuropeptide F in insects[J]. >Journal of Integrative Agriculture, 2020, 19(6): 1429-1438.
[9] NAN Jiu-hong, YIN Li-lin, TANG Zhen-shuang, CHEN Jian-hai, ZHANG Jie, WANG Hai-yan, DU Xiao-yong, LIU Xiang-dong . Genetic parameter estimation and genome-wide association study (GWAS) of red blood cell count at three stages in a Duroc×Erhualian pig population[J]. >Journal of Integrative Agriculture, 2020, 19(3): 793-799.
[10] WANG Li-ning, GAO Wei, WANG Qiong-ying, QU Ji-bin, ZHANG Jin-xia, HUANG Chen-yang. Identification of commercial cultivars of Agaricus bisporus in China using genome-wide microsatellite markers[J]. >Journal of Integrative Agriculture, 2019, 18(3): 580-589.
[11] ZHU Hong, ZHOU Yuan-yuan, ZHAI Hong, HE Shao-zhen, ZHAO Ning, LIU Qing-chang. Transcriptome profiling reveals insights into the molecular mechanism of drought tolerance in sweetpotato[J]. >Journal of Integrative Agriculture, 2019, 18(1): 9-24.
[12] YANG Hai-long, DONG Le, WANG Hui, LIU Chang-lin, LIU Fang, XIE Chuan-xiao. A simple way to visualize detailed phylogenetic tree of huge genomewide SNP data constructed by SNPhylo[J]. >Journal of Integrative Agriculture, 2018, 17(09): 1972-1978.
[13] CHEN Hong-xin, HAN Hai-ming, LI Qing-feng, ZHANG Jin-peng, LU Yu-qing, YANG Xin-ming, LI Xiuquan, LIU Wei-hua, LI Li-hui. Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat[J]. >Journal of Integrative Agriculture, 2018, 17(08): 1697-1705.
[14] WANG Zhen-zhong, XIE Jing-zhong, GUO Li, ZHANG De-yun, LI Gen-qiao, FANG Ti-lin, CHEN Yongxing, LI Jun, WU Qiu-hong, LU Ping, LI Miao-miao, WU Hai-bin, ZHANG Huai-zhi, ZHANG Yan, YANG Wu-yun, LUO Ming. Molecular mapping of YrTZ2, a stripe rust resistance gene in wild emmer accession TZ-2 and its comparative analyses with Aegilops tauschii[J]. >Journal of Integrative Agriculture, 2018, 17(06): 1267-1275.
[15] Syed Adeel Zafar, Amjad Hameed, Muhammad Amjad Nawaz, MA Wei, Mehmood Ali Noor, Muzammil Hussain, Mehboob-ur-Rahman. Mechanisms and molecular approaches for heat tolerance in rice (Oryza sativa L.) under climate change scenario[J]. >Journal of Integrative Agriculture, 2018, 17(04): 726-738.
No Suggested Reading articles found!