水稻蛋白酶抑制剂基因OsLTPL164和OsLTPL151的组成型及诱导型表达模式

doi:10.3864/j.issn.0578-1752.2018.12.008

Abstract

Abstract: 【Objective】Protease inhibitors (PI) is a kind of protein widely existed in plants. It has the function of resisting herbivorous insects feeding. However, the expression patterns of rice (Oryza sativa) PI genes in the feeding process of the Chilo suppressalis are not clear. The objective of this study is to analyze the expression patterns of rice protease inhibitor genes OsLTPL164 and OsLTPL151 under C. suppressalis infestation and mechanical damage, which will lay a theoretical foundation for confirming the function of OsLTPL164 and OsLTPL151 in rice against C. suppressalis infestation and constructing transgenic rice strains using these two genes in the future. 【Method】 Three rice lines, Liaoyan 2 (1654), Liaoxing 17 (1665) and Changbai 17 (1688), which were conventionally planted in Northeast China, were selected as the research materials. Artificial infestation and mechanical injury treatments were performed at the tillering stage of rice, which is the key period of C. suppressalis infestation. The root, stem and leaf of three rice lines were sampled after different times (0, 3, 6, 12, 24, 48, 72 h) of treatments, then the expression level of OsLTPL164 and OsLTPL151 of three rice lines was determined by RT-qPCR. 【Result】The tissue expression pattern of the OsLTPL164 was consistent in three rice lines. The expression level of OsLTPL164 in leaf and stem was higher than that in root. The tissue expression pattern of the OsLTPL151 was not consistent in three rice lines. The expression level of OsLTPL151 in the stem and leaf was significantly higher than in root in the 1654 line, but in the 1688 and 1665 lines, the expression level of OsLTPL151 in root was significantly higher than that in stem and leaf. In addition, the two genes in the same tissue among three rice lines had different expression patterns. The expression level of the OsLTPL164 was the highest in 1665 line, followed by 1688 line, and the lowest in 1654 line. The OsLTPL151 exhibited different expression patterns in different tissues among three rice lines: in the root, the expression level of OsLTPL151 in the 1665 line was significantly higher than that in the 1688 and 1654 lines; in the stem, the expression level of OsLTPL151 in the 1654 and 1665 lines was significantly higher than that in the 1688 line; in the leaf, the expression of OsLTPL151 in the 1654 line was significantly higher than that in the 1665 and 1688 lines. Up-regulation of OsLTPL164 and OsLTPL151 in leaf was more obvious than that of stem and root in the three lines by C. suppressalis infestation, and with a higher inducing effect in 1665 line than that in 1688 and 1654 lines. Both OsLTPL164 and OsLTPL151 showed a rise -decrease-rise expression tendency at different time points after artificial infestation and mechanical injury treatments. The expression level of OsLTPL164 and OsLTPL151 was significantly induced after 6 h of mechanical damage treatment, while the expression level of OsLTPL164 and OsLTPL151 could be significantly induced after 3 h of C. suppressalis infestation. 【Conclusion】The expression of OsLTPL164 and OsLTPL151 in three conventionally grown rice lines can be induced by C. suppressalis infestation and mechanical damage treatment, suggesting that both the two genes are involved in resistance to C. suppressalis. However, other specific functions of the two genes in different rice lines might be different.

Key words: protease inhibitor, Chilo suppressalis, defense, induced expression, RT-qPCR

HE YuJuan, JU Di, WANG Yue, YANG XueQing, WANG XiaoQi . Compositive and Inductive Expression Patterns of Protease Inhibitor Genes OsLTPL164 and OsLTPL151 in Rice (Oryza sativa)[J].Scientia Agricultura Sinica, 2018, 51(12): 2311-2321.

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Compositive and Inductive Expression Patterns of Protease Inhibitor Genes OsLTPL164 and OsLTPL151 in Rice (Oryza sativa)

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