玉米赤霉烯酮对断奶小母猪子宫形态学及热应激蛋白70分布和表达的影响

doi:10.3864/j.issn.0578-1752.2018.04.017

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the effects of zearalenone (ZEA) on uterus morphology, distribution and mRNA relative expression of heat shock protein 70 (HSP 70) in post-weaning piglets. 【Method】 A total of 40 healthy female piglets (Duroc×Landrace×Large White) aged approximately 25-28 d were selected for study. The piglets were transferred to single cages after 10 day adaptation on the obstertric table, then they were allocated into four treatments. Each treatment consisted of 10 replicates of 1 pig per replicate according to the age (35-38) and the average body weight (14.01 ± 0.86 kg). After 10 d of adaptation piglets were fed a normal basal diet supplemented with purified ZEA of 0 (Control), 0.5 (0.52±0.07), 1.0 (1.04±0.03) and 1.5 (1.51±0.13) mg·kg^-1 for 35 d. Animals were housed individually with free access to feed and water. At the end of the feeding trial, piglets were deprived with feed for 12h and sacrificed. Uterus were then isolated under sterile conditions. Two samples of uterus tissues from each pig were quickly collected, one of which was stored at -80℃ to detect relative mRNA expression of HSP70, and the other was fixed promptly in Bouin’s for histological examination and immunohistochemical analysis. 【Result】 Results showed that the myometrium and endometrium became thicker and the number and density of uterine glands increased with the higher level of ZEA. The thickness of myometrium and endometrium increased linearly (P＜0.001) and quadratically (P＜0.001) with higher level of ZEA. The thickness of myometrium and endometrium from ZEA1.5, 1.0 and 0.5 treatments were significantly higher than that of ZEA1.0, 0.5 and control treatments, respectively (P＜0.05). The results of immunohistochemistry indicated that HSP70 immunoreactive substance was mainly distributed in the cytoplasm of uterine smooth muscle cells, uterine glandular epithelial cells, luminal epithelial cells and vascular endothelial cells, but nuclear staining was rarely observed. The light yellow immunereactive substance was shown in the tissues of negative control samples, the color of the positive reaction of HSP70 was enhanced to yellow or brown of block shape with higher level of ZEA. The immunoreactive integrated optic density (IOD) of HSP70 and the total measured part of the uterus in weaning gilts increased linearly (P＜0.001) and quadratically (P＜0.05) in myometrium, luminal epithelium, gland epithelium with rising level of ZEA. In general, the IOD of ZEA1.5 treatment was significantly higher than that of ZEA0.5, while the IOD of ZEA0.5 and ZEA1.0 treatment was significantly higher than that of control. Relative mRNA expression of HSP70 in the uterus of weaning gilts increased linearly (P＜0.05) and quadratically (P＜0.05) with the rising level of ZEA. Although the difference between ZEA1.5 and ZEA1.0 was not significant (P＞0.05), the ZEA1.5 treatment was significantly higher than that of ZEA0.5 treatment (P＜0.05), while ZEA0.5 treatment was significantly higher than that of control (P＜0.05). 【Conclusion】 In conclusion, ZEA at 0.5 mg·kg^-1was sufficient to make the myometrium and endometrium to become thicker, while at the time the concentration could also increase the number of uterine glands and changed the morphology of the uterus; under the condition of this experiment, the positive reaction of HSP70 was enhanced with the higher concentration of ZEA, which means the higher level expression of HSP70 may prevent damage of uterine cells by toxin stress.

Key words: zearalenone, post-weaning piglets, uterus, heat shock protein 70

ZHOU Min, ZHOU XueMei, YANG LiJie, HUANG LiBo, FENG Lei, SHAO MingHui, YANG Chen, YANG WeiRen, YANG ZaiBin, JIANG ShuZhen. Effects of Zearalenone on Expression of Heat Shock Protein 70 and Morphology of Uterus Tissues of Post-Weaning Piglets[J].Scientia Agricultura Sinica, 2018, 51(4): 778-788.

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Effects of Zearalenone on Expression of Heat Shock Protein 70 and Morphology of Uterus Tissues of Post-Weaning Piglets

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