持续淹水对水稻镉吸收的影响及其调控机理

doi:10.3864/j.issn.0578-1752.2017.17.005

Abstract

Abstract: 【Objective】The objective of this experiment is to study the effects of continuous flooding on cadmium (Cd) uptake in rice. By analysis of alteration of soil available Cd, plant Cd content and Cd uptake related gene expression under continuous flooding condition in different Cd accumulation rice varieties, further to reveal the effects and detail regulation mechanism on Cd uptake with continuous flooding management in rice. 【Method】 Pot experiments (exogenous 1.5 mg·kg^-1CdCl₂ ) were carried out by using rice varieties of Fupin36 (FP36, high Cd accumulation) and Zhongjiazao 17 (ZJZ17, low Cd accumulation). Plantlets were treated by continuous flooding management at early tillering stage and sampled at middle tillering stage. Cd content of plant root and shoot, soil available Cd, Fe and Mn content, root plaque Cd, Fe and Mn content and Cd uptake related gene expression of rice were analyzed. The same treatment was continued until mature stage, plantlets and milled rice were harvested to determine Cd content and yield traits. 【Result】 Compared with the control, the results showed that continuous flooding sharply reduced Cd contents of FP36 and ZJZ17 under pollution condition at middle tillering stage, the root Cd content decreased by 39.5% and 33.9%, shoot Cd content decreased by 62.1% and 71.7%, respectively. Continuous flooding also significantly reduced Cd content of rice root, shoot and milled rice at mature stage. The management reduced root, shoot and milled rice Cd content of FP36 by 36.4%, 43.7% and 36.8%, respectively, it also showed a decrease of 62.5%, 61.5% and 55.4% in ZJZ17. Furthermore, the soil available Cd of FP36 and ZJZ17 significantly decreased by 12.1% and 17.7%, and root plaque Cd content decreased by 52.2% and 43.1% under continuous flooding treatment, respectively. While soil available Fe content (23.7% and 10.3%) and Mn content (24.5% and 43.9%) of FP36 and ZJZ17 significantly increased. Root plaque Fe (83.1% and 81.5%) and Mn content (41.5% and 27.7%) were also elevated under continuous flooding. Simultaneously, the relative expression of OsNramp1 (58.3% and 58.0%) and OsLCD (21.6% and 17.8%) genes were also down-regulated by continuous flooding in rice roots.【Conclusion】Continuous flooding decrease Cd uptake by decreasing soil available Cd content and down-regulating Cd uptake genes expression of OsNramp1 and OsLCD in rice.

Key words: rice, cadmium, transport protein, iron, manganese

CHEN JiangMin, YANG YongJie, HUANG QiNa, HU PeiSong, TANG ShaoQing, WU LiQun, WANG JianLong, SHAO GuoSheng. Effects of Continuous Flooding on Cadmium Absorption and Its Regulation Mechanisms in Rice[J].Scientia Agricultura Sinica, 2017, 50(17): 3300-3310.

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Effects of Continuous Flooding on Cadmium Absorption and Its Regulation Mechanisms in Rice

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