油菜株高QTL定位、整合和候选基因鉴定

doi:10.3864/j.issn.0578-1752.2017.17.001

Abstract

Abstract: 【Objective】In order to reveal the genetic architecture and candidate genes for plant height in rapeseed, QTLs were mapped in multiple environments and were integrated with previously reported plant height QTLs and then aligned with the plant height genes, which will provide a basis for the molecular improvement of plant height in rapeseed. 【Method】 The BnaZNF₂population of 184 individuals derived from the elite rapeseed cultivar Zhongshuang11 (de novo sequencing) and No.73290 (re-sequencing) was used as the experimental material. First, the BnaZNF₂ population was subjected to genotype analysis and a high-density linkage map of 803 molecular markers was constructed using Joinmap 4.0. Second, the F_2:3and F_2:4family of BnaZNF₂ population were planted and phenotyped at two locations (Wuhan and Xining) for successive two years (2010 and 2011). Then QTL mapping was conducted by the composite interval mapping method incorporated into WinQTLCart 2.5 software, using the genotype of BnaZNF₂ population and the plant height phenotype of its F_2:3and F_2:4family. 【Result】 After integration of QTLs detected in two locations over two years, a total of 5 consensus QTLs (qPH.A2-1, qPH.A2-2, qPH.C2-1, qPH.C3-1, qPH.C3-2) were obtained, which were distributed on A2, C2 and C3 chromosomes and, explained 2.6%-55.6% of the phenotypic variance. A major QTL on the C2 chromosome, qPH.C2-1, was only detected repeatedly in Xining and its LOD value, additive effect and R²(23.4, -16.0 and 55.6%, respectively) were largest among all of the reported plant height QTLs. Based on the physical map of rapeseed, all of the currently and previously reported plant height QTLs in rapeseed were integrated and then aligned with the plant height genes, which revealed a relatively completed genetic architecture map consisting of 183 QTLs in rapeseed and 287 candidate genes in rapeseed. Of these, a total of 18 QTL cluster were commonly detected in different studies, which were distributed on A1, A2, A3, A6, A7, A9, C6 and C7 chromosomes. In addition, the physical positions of the five QTL detected in the current study were all not overlapped with those of the previously detected plant height QTL, which should be novel. A total of 15 homologues of plant height genes were found within the physical intervals of qPH.A2-2, qPH.C3-1 and qPH.C3-2, of which 11 homologues showed sequence variations between the two parents, which were chosen as the candidates for further study. 【Conclusion】 QTL mapping and integration identified five QTL for plant height in rapeseed, which were all novel. The effect of the major QTL on the C2 chromosome was larger than those of the previously reported plant height QTL, which also showed the strong interaction with the environment. The integration of the reported plant height QTLs and the alignment with the plant height genes systematically revealed the genetic architecture and candidate genes for plant height in rapeseed. By bioinformatics analysis, a total of 11 candidates were identified within the physical intervals of three plant height QTLs detected in the current study.

Key words: Brassica napus L., plant height, genetic architecture, QTL, candidate genes, QTL by environment interaction

ZHANG JiangJiang, ZHAN JiePeng, LIU QingYun, SHI JiaQin, WANG XinFa, LIU GuiHua, WANG HanZhong. QTL Mapping and Integration as well as Candidate Genes Identification for Plant Height in Rapeseed (Brassica napus L.)[J].Scientia Agricultura Sinica, 2017, 50(17): 3247-3258.

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QTL Mapping and Integration as well as Candidate Genes Identification for Plant Height in Rapeseed (Brassica napus L.)

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