中国农业科学 ›› 2012, Vol. 45 ›› Issue (15): 3007-3019.doi: 10.3864/j.issn.0578-1752.2012.15.001
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
叶兴国, 王新敏, 王轲, 杜丽璞, 林志珊, 徐惠君
收稿日期:
2012-03-05
出版日期:
2012-08-01
发布日期:
2012-05-17
通讯作者:
叶兴国,Tel:010-82109765;E-mail:yexg@mail.caas.net.cn。王新敏,E-mail:notebook2005@126.com。叶兴国和王新敏为同等贡献作者
作者简介:
叶兴国,Tel:010-82109765;E-mail:yexg@mail.caas.net.cn。王新敏,E-mail:notebook2005@126.com。叶兴国和王新敏为同等贡献作者
基金资助:
国家转基因研究专项(2011ZX08010-004)
YE Xing-Guo, WANG Xin-Min, WANG Ke, DU Li-Pu, LIN Zhi-Shan, XU Hui-Jun
Received:
2012-03-05
Online:
2012-08-01
Published:
2012-05-17
摘要: 农杆菌介导是目前转基因植物研究采用的主要方法,不同植物间利用农杆菌转化的转化效率差异很大,高效农杆菌转化体系对于转基因植物产业化和功能基因组学等研究具有重要意义。总体而言,影响农杆菌转化效率的因素主要包括基因型、外植体及其生理状态、农杆菌菌株、培养基成分、共培养条件等。对于一些难转化的植物来说,辅助因素对转化效率的提高起着至关重要的作用。本文综述了影响农杆菌转化效率的一些辅助因素及其作用,包括植物材料微创伤处理和干燥处理、培养基中抗氧化剂和表面活性剂使用、农杆菌中额外拷贝Vir和植物细胞中VIP(VirE2 interacting protein)过表达、载体上核基质附着序列引入等,以期为进一步改进难转化作物如小麦、大豆等农杆菌介导的遗传转化效率提供参考。
叶兴国, 王新敏, 王轲, 杜丽璞, 林志珊, 徐惠君. 提高植物农杆菌转化效率辅助策略研究进展[J]. 中国农业科学, 2012, 45(15): 3007-3019.
YE Xing-Guo, WANG Xin-Min, WANG Ke, DU Li-Pu, LIN Zhi-Shan, XU Hui-Jun. A Review of Some Assisted Strategies for Improving the Efficiency of Agrobacterium-Mediated Plant Transformation[J]. Scientia Agricultura Sinica, 2012, 45(15): 3007-3019.
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