中国农业科学 ›› 2013, Vol. 46 ›› Issue (2): 367-375.doi: 10.3864/j.issn.0578-1752.2013.02.016

• 畜牧·资源昆虫 • 上一篇    下一篇

黄牛、牦牛和犏牛b-Sycp2基因的克隆 与睾丸组织mRNA的表达水平

 骆骅, 贾超, 屈旭光, 赵兴波, 钟金城, 谢庄, 李齐发   

  1. 1.南京农业大学动物科技学院,南京 210095
    2.中国农业大学动物科技学院,北京 100193
    3.西南民族大学生命科学与技术学院,成都 610041
  • 收稿日期:2012-05-11 出版日期:2013-01-15 发布日期:2012-07-03
  • 通讯作者: 通信作者李齐发,E-mail:liqifa@njau.edu.cn
  • 作者简介:骆骅,E-mail:2010105013@njau.edu.cn。贾超,E-mail:jiachao@njau.edu.cn。骆骅与贾超为同等贡献作者。
  • 基金资助:

    国家自然科学基金项目(30500360)、农业生物技术国家重点实验室开放课题(2009SKLAB07-2)

Cloning and Testicular Expression Level of b-Sycp2 in Cattle, Yak and Cattle-Yak

 LUO  Hua, JIA  Chao, QU  Xu-Guang, ZHAO  Xing-Bo, ZHONG  Jin-Cheng, XIE  Zhuang, LI  Qi-Fa   

  1. 1.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095
    2.College of Animal Science and Technology, China Agricultural University, Beijing 100193
    3.College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041
  • Received:2012-05-11 Online:2013-01-15 Published:2012-07-03

摘要: 【目的】克隆黄牛、牦牛和犏牛Sycp2基因序列,了解牛Sycp2基因序列特征和组织表达特征,分析睾丸组织中Sycp2基因的表达水平。【方法】采用电子克隆和克隆测序技术获得黄牛、牦牛和犏牛Sycp2基因序列,利用生物信息学方法分析其序列特征;采用RT-PCR分析牛Sycp2基因的组织表达特征;采用real-time PCR技术检测黄牛、牦牛和犏牛睾丸组织Sycp2基因的表达水平。【结果】①黄牛、牦牛和犏牛Sycp2基因编码区序列全长均为4 365 bp,命名为b-Sycp2,编码蛋白含有1 454个氨基酸残基,并包含卷曲螺旋结构域等典型结构域;②b-Sycp2基因在睾丸组织中特异表达,黄牛和牦牛睾丸组织中b-Sycp2基因的表达水平显著高于犏牛(P<0.05)。【结论】成功克隆了b-Sycp2基因,b-Sycp2基因为睾丸组织的特异表达基因,且黄牛和牦牛睾丸组织b-Sycp2基因表达水平显著高于犏牛。

关键词: 黄牛 , 牦牛 , 犏牛 , b-Sycp2基因 , 克隆 , 表达水平

Abstract: 【Objective】The study was aimed to clone the Sycp2 gene sequence of cattle, yak and cattle-yak, investigate the gene structures and its tissue-expression patterns and testicular expression levels in cattle, yak and cattle-yak.【Method】 In silico cloning and clone sequencing were applied to acquire the coding region sequences of Sycp2 gene of cattle, yak and yak-cattle, and molecular characterization were analyzed by bioinformatics software. RT-PCR was applied to analyze the tissue-expression patterns, and real-time PCR was employed to examine the expression levels in cattle, yak and cattle-yak testis.【Result】The full length of the coding region sequences of the Sycp2 gene was 4 365 bp, named b-Sycp2. The b-Sycp2 gene in cattle, yak and cattle-yak encoded 1 454 amino acid residues which included some typical domains such as coiled-coil domain. RT-PCR analysis showed that b-Sycp2 gene was expressed only in the testis. Real-time PCR analysis revealed that the mRNA expression level of b-Sycp2 gene in the testis of cattle and yak was remarkably higher than that of in cattle-yak (P<0.05). 【Conclusion】The b-Sycp2 gene was cloned successfully, and it expressed only in testis, b-Sycp2 mRNA expression level of cattle and yak represented a dramatic higher than that of cattle-yak.

Key words: cattle , yak , cattle-yak , b-Sycp2 gene , clone , expression level